Application of the 50% Hydrazine Solution Method for O-Glycans Release, their Chemical Labeling, and HPLC Separation

Mucins are high molecular mass glycoproteins with oligosaccharides O-bonded to the protein core. β-elimination is the most popular method used for releasing of O-glycans. However to such released glycoforms it is difficult to introduce a label to amplify a signal for oligosaccharide detection. In ou...

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Autores principales: Kisiel, D. G., Radziejewska, I., Gindzieński, A.
Formato: Texto
Lenguaje:English
Publicado: Informa Healthcare 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2728758/
https://www.ncbi.nlm.nih.gov/pubmed/19710917
http://dx.doi.org/10.1080/15376510701623755
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author Kisiel, D. G.
Radziejewska, I.
Gindzieński, A.
author_facet Kisiel, D. G.
Radziejewska, I.
Gindzieński, A.
author_sort Kisiel, D. G.
collection PubMed
description Mucins are high molecular mass glycoproteins with oligosaccharides O-bonded to the protein core. β-elimination is the most popular method used for releasing of O-glycans. However to such released glycoforms it is difficult to introduce a label to amplify a signal for oligosaccharide detection. In our study we used a combination of the β-elimination and hydrazinolysis methods. Released glycoforms were labeled with para-amino benzooic acid ethyl ester (ABBE) and fractionated on HPLC column. This combined procedure seems to be a good tool for O-glycans analysis.
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spelling pubmed-27287582009-08-25 Application of the 50% Hydrazine Solution Method for O-Glycans Release, their Chemical Labeling, and HPLC Separation Kisiel, D. G. Radziejewska, I. Gindzieński, A. Toxicol Mech Methods Article Mucins are high molecular mass glycoproteins with oligosaccharides O-bonded to the protein core. β-elimination is the most popular method used for releasing of O-glycans. However to such released glycoforms it is difficult to introduce a label to amplify a signal for oligosaccharide detection. In our study we used a combination of the β-elimination and hydrazinolysis methods. Released glycoforms were labeled with para-amino benzooic acid ethyl ester (ABBE) and fractionated on HPLC column. This combined procedure seems to be a good tool for O-glycans analysis. Informa Healthcare 2008-06-23 2008-07 /pmc/articles/PMC2728758/ /pubmed/19710917 http://dx.doi.org/10.1080/15376510701623755 Text en Copyright © Informa Healthcare USA, Inc. http://creativecommons.org/licenses/by/2.0/ This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Informa Healthcare journals (http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Kisiel, D. G.
Radziejewska, I.
Gindzieński, A.
Application of the 50% Hydrazine Solution Method for O-Glycans Release, their Chemical Labeling, and HPLC Separation
title Application of the 50% Hydrazine Solution Method for O-Glycans Release, their Chemical Labeling, and HPLC Separation
title_full Application of the 50% Hydrazine Solution Method for O-Glycans Release, their Chemical Labeling, and HPLC Separation
title_fullStr Application of the 50% Hydrazine Solution Method for O-Glycans Release, their Chemical Labeling, and HPLC Separation
title_full_unstemmed Application of the 50% Hydrazine Solution Method for O-Glycans Release, their Chemical Labeling, and HPLC Separation
title_short Application of the 50% Hydrazine Solution Method for O-Glycans Release, their Chemical Labeling, and HPLC Separation
title_sort application of the 50% hydrazine solution method for o-glycans release, their chemical labeling, and hplc separation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2728758/
https://www.ncbi.nlm.nih.gov/pubmed/19710917
http://dx.doi.org/10.1080/15376510701623755
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