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High-Performance Liquid Chromatography Method for the Determination of Folic Acid in Fortified Food Products

Reversed-phase high-performance liquid chromatography, coupled with coulometric electrochemical detection, was successfully applied for the quantification of added folic acid (FA) in fortified fruit juices and cereal products. The method allowed good separation of the 5-HCO-H(4) folate and folic aci...

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Autores principales: Lebiedzińska, A., Da̧browska, M., Szefer, P., Marszałł, M.
Formato: Texto
Lenguaje:English
Publicado: Informa Healthcare 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2728762/
https://www.ncbi.nlm.nih.gov/pubmed/19696945
http://dx.doi.org/10.1080/15376510701623870
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author Lebiedzińska, A.
Da̧browska, M.
Szefer, P.
Marszałł, M.
author_facet Lebiedzińska, A.
Da̧browska, M.
Szefer, P.
Marszałł, M.
author_sort Lebiedzińska, A.
collection PubMed
description Reversed-phase high-performance liquid chromatography, coupled with coulometric electrochemical detection, was successfully applied for the quantification of added folic acid (FA) in fortified fruit juices and cereal products. The method allowed good separation of the 5-HCO-H(4) folate and folic acid in cereal samples. The retention times of vitamins were repeatedly determined by isocratic elution using 40 mM sodium phosphate dibasic, heptahydrate buffer, and 8% acetonitrile (v/v) (0.9 mL/min, pH 5.5) as mobile phase with the Supelco LC 18 column 5 μm (25 cm × 4.6 mm). Folate concentrations were measured using a trienzyme (hog kidney folate conjugase, α-amylase, and protease) folate extraction method.
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spelling pubmed-27287622009-08-19 High-Performance Liquid Chromatography Method for the Determination of Folic Acid in Fortified Food Products Lebiedzińska, A. Da̧browska, M. Szefer, P. Marszałł, M. Toxicol Mech Methods Article Reversed-phase high-performance liquid chromatography, coupled with coulometric electrochemical detection, was successfully applied for the quantification of added folic acid (FA) in fortified fruit juices and cereal products. The method allowed good separation of the 5-HCO-H(4) folate and folic acid in cereal samples. The retention times of vitamins were repeatedly determined by isocratic elution using 40 mM sodium phosphate dibasic, heptahydrate buffer, and 8% acetonitrile (v/v) (0.9 mL/min, pH 5.5) as mobile phase with the Supelco LC 18 column 5 μm (25 cm × 4.6 mm). Folate concentrations were measured using a trienzyme (hog kidney folate conjugase, α-amylase, and protease) folate extraction method. Informa Healthcare 2008-06-23 2008-07 /pmc/articles/PMC2728762/ /pubmed/19696945 http://dx.doi.org/10.1080/15376510701623870 Text en Copyright © Informa Healthcare USA, Inc. http://creativecommons.org/licenses/by/2.0/ This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Informa Healthcare journals (http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Lebiedzińska, A.
Da̧browska, M.
Szefer, P.
Marszałł, M.
High-Performance Liquid Chromatography Method for the Determination of Folic Acid in Fortified Food Products
title High-Performance Liquid Chromatography Method for the Determination of Folic Acid in Fortified Food Products
title_full High-Performance Liquid Chromatography Method for the Determination of Folic Acid in Fortified Food Products
title_fullStr High-Performance Liquid Chromatography Method for the Determination of Folic Acid in Fortified Food Products
title_full_unstemmed High-Performance Liquid Chromatography Method for the Determination of Folic Acid in Fortified Food Products
title_short High-Performance Liquid Chromatography Method for the Determination of Folic Acid in Fortified Food Products
title_sort high-performance liquid chromatography method for the determination of folic acid in fortified food products
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2728762/
https://www.ncbi.nlm.nih.gov/pubmed/19696945
http://dx.doi.org/10.1080/15376510701623870
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