Construction of a Recombinant Eukaryotic Expression Plasmid Containing Human Calcitonin Gene and Its Expression in NIH3T3 Cells

Aim. To construct a recombinant eukaryotic expression plasmid containing human calcitonin (hCT) gene and express the gene in murine fibroblast NIH3T3 cells. Materials and Methods. A murine Igκ-chain leader sequence and hCT gene were synthesized and cloned into pCDNA3.0 to form the pCDNA3.0-Igκ-hCT e...

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Detalles Bibliográficos
Autores principales: Li, Xiaolin, Jiang, Guozhong, Wu, Dan, Wang, Xiuli, Zeng, Bingfang
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729102/
https://www.ncbi.nlm.nih.gov/pubmed/19696904
http://dx.doi.org/10.1155/2009/241390

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729102/
https://www.ncbi.nlm.nih.gov/pubmed/19696904
http://dx.doi.org/10.1155/2009/241390

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