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Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells
Several reports have documented the derivation of pluripotent cells (multipotent germline stem cells) from spermatogonial stem cells obtained from the adult mouse testis. These spermatogonia-derived stem cells express embryonic stem cell markers and differentiate to the three primary germ layers, as...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Wiley Subscription Services, Inc., A Wiley Company
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729695/ https://www.ncbi.nlm.nih.gov/pubmed/18927477 http://dx.doi.org/10.1634/stemcells.2008-0439 |
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author | Kossack, Nina Meneses, Juanito Shefi, Shai Nguyen, Ha Nam Chavez, Shawn Nicholas, Cory Gromoll, Joerg Turek, Paul J Reijo-Pera, Renee A |
author_facet | Kossack, Nina Meneses, Juanito Shefi, Shai Nguyen, Ha Nam Chavez, Shawn Nicholas, Cory Gromoll, Joerg Turek, Paul J Reijo-Pera, Renee A |
author_sort | Kossack, Nina |
collection | PubMed |
description | Several reports have documented the derivation of pluripotent cells (multipotent germline stem cells) from spermatogonial stem cells obtained from the adult mouse testis. These spermatogonia-derived stem cells express embryonic stem cell markers and differentiate to the three primary germ layers, as well as the germline. Data indicate that derivation may involve reprogramming of endogenous spermatogonia in culture. Here, we report the derivation of human multipotent germline stem cells (hMGSCs) from a testis biopsy. The cells express distinct markers of pluripotency, form embryoid bodies that contain derivatives of all three germ layers, maintain a normal XY karyotype, are hypomethylated at the H19 locus, and express high levels of telomerase. Teratoma assays indicate the presence of human cells 8 weeks post-transplantation but limited teratoma formation. Thus, these data suggest the potential to derive pluripotent cells from human testis biopsies but indicate a need for novel strategies to optimize hMGSC culture conditions and reprogramming. |
format | Text |
id | pubmed-2729695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Wiley Subscription Services, Inc., A Wiley Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-27296952009-08-27 Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells Kossack, Nina Meneses, Juanito Shefi, Shai Nguyen, Ha Nam Chavez, Shawn Nicholas, Cory Gromoll, Joerg Turek, Paul J Reijo-Pera, Renee A Stem Cells Tissue-Specific Stem Cells Several reports have documented the derivation of pluripotent cells (multipotent germline stem cells) from spermatogonial stem cells obtained from the adult mouse testis. These spermatogonia-derived stem cells express embryonic stem cell markers and differentiate to the three primary germ layers, as well as the germline. Data indicate that derivation may involve reprogramming of endogenous spermatogonia in culture. Here, we report the derivation of human multipotent germline stem cells (hMGSCs) from a testis biopsy. The cells express distinct markers of pluripotency, form embryoid bodies that contain derivatives of all three germ layers, maintain a normal XY karyotype, are hypomethylated at the H19 locus, and express high levels of telomerase. Teratoma assays indicate the presence of human cells 8 weeks post-transplantation but limited teratoma formation. Thus, these data suggest the potential to derive pluripotent cells from human testis biopsies but indicate a need for novel strategies to optimize hMGSC culture conditions and reprogramming. Wiley Subscription Services, Inc., A Wiley Company 2009-01 /pmc/articles/PMC2729695/ /pubmed/18927477 http://dx.doi.org/10.1634/stemcells.2008-0439 Text en Copyright © 2009 AlphaMed Press http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Tissue-Specific Stem Cells Kossack, Nina Meneses, Juanito Shefi, Shai Nguyen, Ha Nam Chavez, Shawn Nicholas, Cory Gromoll, Joerg Turek, Paul J Reijo-Pera, Renee A Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells |
title | Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells |
title_full | Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells |
title_fullStr | Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells |
title_full_unstemmed | Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells |
title_short | Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells |
title_sort | isolation and characterization of pluripotent human spermatogonial stem cell-derived cells |
topic | Tissue-Specific Stem Cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729695/ https://www.ncbi.nlm.nih.gov/pubmed/18927477 http://dx.doi.org/10.1634/stemcells.2008-0439 |
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