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B Cells in Murine Cervical Lymph Nodes are Conventional B-2 Cells
We investigated the characteristic features of cervical lymph node B cells to determine whether their behavior differs from that of B cells located elsewhere, because cervical lymph nodes may be exposed to continual antigenic stimulation from the naso- and/or oropharynx. B cells were isolated from c...
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Formato: | Texto |
Lenguaje: | English |
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The Korean Academy of Medical Sciences
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729940/ https://www.ncbi.nlm.nih.gov/pubmed/16778378 http://dx.doi.org/10.3346/jkms.2006.21.3.391 |
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author | Yeo, Seung Geun Cho, Joong Saeng Park, Dong Choon |
author_facet | Yeo, Seung Geun Cho, Joong Saeng Park, Dong Choon |
author_sort | Yeo, Seung Geun |
collection | PubMed |
description | We investigated the characteristic features of cervical lymph node B cells to determine whether their behavior differs from that of B cells located elsewhere, because cervical lymph nodes may be exposed to continual antigenic stimulation from the naso- and/or oropharynx. B cells were isolated from cervical lymph nodes, spleen and peritoneal fluid of mice, cultured in medium, and exposed to various stimuli. The expression of various surface molecules characteristic of lymphoid B cells was assayed by flow cytometry, and immunoglobulin secreted into the culture supernatants was evaluated by enzyme-linked immunosorbent assay. B220+ cells were cultured in medium alone or with lipopolysaccharide, and their entrance into S phase in response to stimuli was measured by proliferative assays. Phenotypic characteristics of cervical lymph node B cells included CD5(low), CD23(high), CD43(low), B7.1(low), B7.2(low), and Syndecan-1(low). Unstimulated lymphoid B cells did not secrete immunoglobulin, but, upon stimulation, secretion of IgM was increased more than secretion of IgA and IgG. B cells actively entered S phase after 48 hr stimulation. These results show that B cells in cervical lymph nodes are conventional B2 cells, like splenic B cells. |
format | Text |
id | pubmed-2729940 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Korean Academy of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-27299402009-08-24 B Cells in Murine Cervical Lymph Nodes are Conventional B-2 Cells Yeo, Seung Geun Cho, Joong Saeng Park, Dong Choon J Korean Med Sci Original Article We investigated the characteristic features of cervical lymph node B cells to determine whether their behavior differs from that of B cells located elsewhere, because cervical lymph nodes may be exposed to continual antigenic stimulation from the naso- and/or oropharynx. B cells were isolated from cervical lymph nodes, spleen and peritoneal fluid of mice, cultured in medium, and exposed to various stimuli. The expression of various surface molecules characteristic of lymphoid B cells was assayed by flow cytometry, and immunoglobulin secreted into the culture supernatants was evaluated by enzyme-linked immunosorbent assay. B220+ cells were cultured in medium alone or with lipopolysaccharide, and their entrance into S phase in response to stimuli was measured by proliferative assays. Phenotypic characteristics of cervical lymph node B cells included CD5(low), CD23(high), CD43(low), B7.1(low), B7.2(low), and Syndecan-1(low). Unstimulated lymphoid B cells did not secrete immunoglobulin, but, upon stimulation, secretion of IgM was increased more than secretion of IgA and IgG. B cells actively entered S phase after 48 hr stimulation. These results show that B cells in cervical lymph nodes are conventional B2 cells, like splenic B cells. The Korean Academy of Medical Sciences 2006-06 2006-06-21 /pmc/articles/PMC2729940/ /pubmed/16778378 http://dx.doi.org/10.3346/jkms.2006.21.3.391 Text en Copyright © 2006 The Korean Academy of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Yeo, Seung Geun Cho, Joong Saeng Park, Dong Choon B Cells in Murine Cervical Lymph Nodes are Conventional B-2 Cells |
title | B Cells in Murine Cervical Lymph Nodes are Conventional B-2 Cells |
title_full | B Cells in Murine Cervical Lymph Nodes are Conventional B-2 Cells |
title_fullStr | B Cells in Murine Cervical Lymph Nodes are Conventional B-2 Cells |
title_full_unstemmed | B Cells in Murine Cervical Lymph Nodes are Conventional B-2 Cells |
title_short | B Cells in Murine Cervical Lymph Nodes are Conventional B-2 Cells |
title_sort | b cells in murine cervical lymph nodes are conventional b-2 cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729940/ https://www.ncbi.nlm.nih.gov/pubmed/16778378 http://dx.doi.org/10.3346/jkms.2006.21.3.391 |
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