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Long-term performance and microbial community analysis of a full-scale synthesis gas fed reactor treating sulfate- and zinc-rich wastewater

The performance of a full-scale (500 m(3)) sulfidogenic synthesis gas fed gas-lift reactor treating metal- and sulfate-rich wastewater was investigated over a period of 128 weeks. After startup, the reactor had a high methanogenic activity of 46 Nm(3)·h(−1). Lowering the carbon dioxide feed rate dur...

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Autores principales: van Houten, Bernd H. G. W., van Doesburg, Wim, Dijkman, Henk, Copini, Cris, Smidt, Hauke, Stams, Alfons J. M.
Formato: Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729981/
https://www.ncbi.nlm.nih.gov/pubmed/19543724
http://dx.doi.org/10.1007/s00253-009-2075-8
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author van Houten, Bernd H. G. W.
van Doesburg, Wim
Dijkman, Henk
Copini, Cris
Smidt, Hauke
Stams, Alfons J. M.
author_facet van Houten, Bernd H. G. W.
van Doesburg, Wim
Dijkman, Henk
Copini, Cris
Smidt, Hauke
Stams, Alfons J. M.
author_sort van Houten, Bernd H. G. W.
collection PubMed
description The performance of a full-scale (500 m(3)) sulfidogenic synthesis gas fed gas-lift reactor treating metal- and sulfate-rich wastewater was investigated over a period of 128 weeks. After startup, the reactor had a high methanogenic activity of 46 Nm(3)·h(−1). Lowering the carbon dioxide feed rate during the first 6 weeks gradually lowered the methane production rate. Between weeks 8 and 93, less than 1% of the hydrogen supplied was used for methanogenesis. Denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified 16S rRNA gene fragments showed that the archaeal community decreased in diversity but did not disappear completely. After the carbon dioxide feed rate increased in week 88, the methane production rate also increased, confirming that methane production was carbon dioxide limited. Even though lowering the carbon dioxide feed appeared to affect part of the sulfate-reducing community, it did not prevent achieving the desired rates of sulfate reduction. The average sulfate conversion rate was 181 kg∙h(−1) for the first 92 weeks. After 92 weeks, the sulfate input rate was increased and from week 94 to 128, the average weekly sulfate conversion rate was 295 kg·h(−1) (SD ± 87). Even higher sulfate conversion rates of up to 400 kg·h(−1) could be sustained for weeks 120–128. The long-term performance and stability together with the ability to control methanogenesis demonstrates that synthesis gas fed reactor can be used successfully at full scale to treat metal and sulfate-rich wastewater.
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spelling pubmed-27299812009-08-24 Long-term performance and microbial community analysis of a full-scale synthesis gas fed reactor treating sulfate- and zinc-rich wastewater van Houten, Bernd H. G. W. van Doesburg, Wim Dijkman, Henk Copini, Cris Smidt, Hauke Stams, Alfons J. M. Appl Microbiol Biotechnol Environmental Biotechnology The performance of a full-scale (500 m(3)) sulfidogenic synthesis gas fed gas-lift reactor treating metal- and sulfate-rich wastewater was investigated over a period of 128 weeks. After startup, the reactor had a high methanogenic activity of 46 Nm(3)·h(−1). Lowering the carbon dioxide feed rate during the first 6 weeks gradually lowered the methane production rate. Between weeks 8 and 93, less than 1% of the hydrogen supplied was used for methanogenesis. Denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified 16S rRNA gene fragments showed that the archaeal community decreased in diversity but did not disappear completely. After the carbon dioxide feed rate increased in week 88, the methane production rate also increased, confirming that methane production was carbon dioxide limited. Even though lowering the carbon dioxide feed appeared to affect part of the sulfate-reducing community, it did not prevent achieving the desired rates of sulfate reduction. The average sulfate conversion rate was 181 kg∙h(−1) for the first 92 weeks. After 92 weeks, the sulfate input rate was increased and from week 94 to 128, the average weekly sulfate conversion rate was 295 kg·h(−1) (SD ± 87). Even higher sulfate conversion rates of up to 400 kg·h(−1) could be sustained for weeks 120–128. The long-term performance and stability together with the ability to control methanogenesis demonstrates that synthesis gas fed reactor can be used successfully at full scale to treat metal and sulfate-rich wastewater. Springer Berlin Heidelberg 2009-06-19 2009 /pmc/articles/PMC2729981/ /pubmed/19543724 http://dx.doi.org/10.1007/s00253-009-2075-8 Text en © The Author(s) 2009 Open AccessThis is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Environmental Biotechnology
van Houten, Bernd H. G. W.
van Doesburg, Wim
Dijkman, Henk
Copini, Cris
Smidt, Hauke
Stams, Alfons J. M.
Long-term performance and microbial community analysis of a full-scale synthesis gas fed reactor treating sulfate- and zinc-rich wastewater
title Long-term performance and microbial community analysis of a full-scale synthesis gas fed reactor treating sulfate- and zinc-rich wastewater
title_full Long-term performance and microbial community analysis of a full-scale synthesis gas fed reactor treating sulfate- and zinc-rich wastewater
title_fullStr Long-term performance and microbial community analysis of a full-scale synthesis gas fed reactor treating sulfate- and zinc-rich wastewater
title_full_unstemmed Long-term performance and microbial community analysis of a full-scale synthesis gas fed reactor treating sulfate- and zinc-rich wastewater
title_short Long-term performance and microbial community analysis of a full-scale synthesis gas fed reactor treating sulfate- and zinc-rich wastewater
title_sort long-term performance and microbial community analysis of a full-scale synthesis gas fed reactor treating sulfate- and zinc-rich wastewater
topic Environmental Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729981/
https://www.ncbi.nlm.nih.gov/pubmed/19543724
http://dx.doi.org/10.1007/s00253-009-2075-8
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