Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis

A two-component direct fluorescent-antibody (DFA) assay, using fluorescein-labeled monoclonal antibodies specific to the Bacillus anthracis cell wall (CW-DFA) and capsule (CAP-DFA) antigens, was evaluated and validated for rapid identification of B. anthracis. We analyzed 230 B. anthracis isolates;...

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Autores principales: De, Barun K., Bragg, Sandra L., Sanden, Gary N., Wilson, Kathy E., Diem, Lois A., Marston, Chung K., Hoffmaster, Alex R., Barnet, Gwen A., Weyant, Robbin S., Abshire, Teresa G., Ezzell, John W., Popovic, Tanja
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2002
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2730293/
https://www.ncbi.nlm.nih.gov/pubmed/12396916
http://dx.doi.org/10.3201/eid0810.020392
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author De, Barun K.
Bragg, Sandra L.
Sanden, Gary N.
Wilson, Kathy E.
Diem, Lois A.
Marston, Chung K.
Hoffmaster, Alex R.
Barnet, Gwen A.
Weyant, Robbin S.
Abshire, Teresa G.
Ezzell, John W.
Popovic, Tanja
author_facet De, Barun K.
Bragg, Sandra L.
Sanden, Gary N.
Wilson, Kathy E.
Diem, Lois A.
Marston, Chung K.
Hoffmaster, Alex R.
Barnet, Gwen A.
Weyant, Robbin S.
Abshire, Teresa G.
Ezzell, John W.
Popovic, Tanja
author_sort De, Barun K.
collection PubMed
description A two-component direct fluorescent-antibody (DFA) assay, using fluorescein-labeled monoclonal antibodies specific to the Bacillus anthracis cell wall (CW-DFA) and capsule (CAP-DFA) antigens, was evaluated and validated for rapid identification of B. anthracis. We analyzed 230 B. anthracis isolates; 228 and 229 were positive by CW-DFA and CAP-DFA assays, respectively. We also tested 56 non–B. anthracis strains; 10 B. cereus and 2 B. thuringiensis were positive by the CW-DFA assay, and 1 B. megaterium strain was positive by CAP-DFA. Analysis of the combined DFA results identified 227 of 230 B. anthracis isolates; all 56 strains of the other Bacillus spp. were negative. Both DFA assays tested positive on 14 of 26 clinical specimens from the 2001 anthrax outbreak investigation. The two-component DFA assay is a sensitive, specific, and rapid confirmatory test for B. anthracis in cultures and may be useful directly on clinical specimens.
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spelling pubmed-27302932009-09-16 Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis De, Barun K. Bragg, Sandra L. Sanden, Gary N. Wilson, Kathy E. Diem, Lois A. Marston, Chung K. Hoffmaster, Alex R. Barnet, Gwen A. Weyant, Robbin S. Abshire, Teresa G. Ezzell, John W. Popovic, Tanja Emerg Infect Dis Dispatch A two-component direct fluorescent-antibody (DFA) assay, using fluorescein-labeled monoclonal antibodies specific to the Bacillus anthracis cell wall (CW-DFA) and capsule (CAP-DFA) antigens, was evaluated and validated for rapid identification of B. anthracis. We analyzed 230 B. anthracis isolates; 228 and 229 were positive by CW-DFA and CAP-DFA assays, respectively. We also tested 56 non–B. anthracis strains; 10 B. cereus and 2 B. thuringiensis were positive by the CW-DFA assay, and 1 B. megaterium strain was positive by CAP-DFA. Analysis of the combined DFA results identified 227 of 230 B. anthracis isolates; all 56 strains of the other Bacillus spp. were negative. Both DFA assays tested positive on 14 of 26 clinical specimens from the 2001 anthrax outbreak investigation. The two-component DFA assay is a sensitive, specific, and rapid confirmatory test for B. anthracis in cultures and may be useful directly on clinical specimens. Centers for Disease Control and Prevention 2002-10 /pmc/articles/PMC2730293/ /pubmed/12396916 http://dx.doi.org/10.3201/eid0810.020392 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
De, Barun K.
Bragg, Sandra L.
Sanden, Gary N.
Wilson, Kathy E.
Diem, Lois A.
Marston, Chung K.
Hoffmaster, Alex R.
Barnet, Gwen A.
Weyant, Robbin S.
Abshire, Teresa G.
Ezzell, John W.
Popovic, Tanja
Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis
title Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis
title_full Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis
title_fullStr Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis
title_full_unstemmed Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis
title_short Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis
title_sort two-component direct fluorescent-antibody assay for rapid identification of bacillus anthracis
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2730293/
https://www.ncbi.nlm.nih.gov/pubmed/12396916
http://dx.doi.org/10.3201/eid0810.020392
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