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Immunohistochemical Localisation of PDE5 in Rat Lung during Pre- and Postnatal Development
In mammalian lung, at the transition to extrauterine life, NO/cGMP signal transduction system is known to play crucial roles in the regulation of vascular resistance and is supposed to act in angiogenesis. PDE5, which is the most abundant cGMP metabolizing enzyme within the lung, is highly expressed...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2730472/ https://www.ncbi.nlm.nih.gov/pubmed/19707527 http://dx.doi.org/10.1155/2009/932961 |
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author | Scipioni, Angela Giorgi, Mauro Nuccetelli, Valeria Stefanini, Stefania |
author_facet | Scipioni, Angela Giorgi, Mauro Nuccetelli, Valeria Stefanini, Stefania |
author_sort | Scipioni, Angela |
collection | PubMed |
description | In mammalian lung, at the transition to extrauterine life, NO/cGMP signal transduction system is known to play crucial roles in the regulation of vascular resistance and is supposed to act in angiogenesis. PDE5, which is the most abundant cGMP metabolizing enzyme within the lung, is highly expressed in the perinatal period, but its localisation in the different pulmonary cells is still poorly known. In our research, PDE5 immunohistochemical distribution was investigated in foetal and neonatal rat lung. The highest expression of PDE5 was found in cells randomly located in the stroma; in newborns, in particular, many cells in the intersaccular walls were heavily labelled, while much lower staining levels were shown by smooth myocytes belonging to vessels and airways. On the basis of their immunoreactivity for α-SM actin and/or desmin, most of the heavily PDE5-positive cells were identified as interstitial myofibroblasts and transitional pericytes, while only a few were interpreted as interstitial lipofibroblasts. |
format | Text |
id | pubmed-2730472 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-27304722009-08-25 Immunohistochemical Localisation of PDE5 in Rat Lung during Pre- and Postnatal Development Scipioni, Angela Giorgi, Mauro Nuccetelli, Valeria Stefanini, Stefania J Biomed Biotechnol Research Article In mammalian lung, at the transition to extrauterine life, NO/cGMP signal transduction system is known to play crucial roles in the regulation of vascular resistance and is supposed to act in angiogenesis. PDE5, which is the most abundant cGMP metabolizing enzyme within the lung, is highly expressed in the perinatal period, but its localisation in the different pulmonary cells is still poorly known. In our research, PDE5 immunohistochemical distribution was investigated in foetal and neonatal rat lung. The highest expression of PDE5 was found in cells randomly located in the stroma; in newborns, in particular, many cells in the intersaccular walls were heavily labelled, while much lower staining levels were shown by smooth myocytes belonging to vessels and airways. On the basis of their immunoreactivity for α-SM actin and/or desmin, most of the heavily PDE5-positive cells were identified as interstitial myofibroblasts and transitional pericytes, while only a few were interpreted as interstitial lipofibroblasts. Hindawi Publishing Corporation 2009 2009-08-20 /pmc/articles/PMC2730472/ /pubmed/19707527 http://dx.doi.org/10.1155/2009/932961 Text en Copyright © 2009 Angela Scipioni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Scipioni, Angela Giorgi, Mauro Nuccetelli, Valeria Stefanini, Stefania Immunohistochemical Localisation of PDE5 in Rat Lung during Pre- and Postnatal Development |
title | Immunohistochemical Localisation of PDE5 in Rat Lung during Pre- and Postnatal Development |
title_full | Immunohistochemical Localisation of PDE5 in Rat Lung during Pre- and Postnatal Development |
title_fullStr | Immunohistochemical Localisation of PDE5 in Rat Lung during Pre- and Postnatal Development |
title_full_unstemmed | Immunohistochemical Localisation of PDE5 in Rat Lung during Pre- and Postnatal Development |
title_short | Immunohistochemical Localisation of PDE5 in Rat Lung during Pre- and Postnatal Development |
title_sort | immunohistochemical localisation of pde5 in rat lung during pre- and postnatal development |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2730472/ https://www.ncbi.nlm.nih.gov/pubmed/19707527 http://dx.doi.org/10.1155/2009/932961 |
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