Engineering and Directed Evolution of a Ca(2+) Binding Site A-Deficient AprE Mutant Reveal an Essential Contribution of the Loop Leu(75)–Leu(82) to Enzyme Activity

An aprE mutant from B. subtilis 168 lacking the connecting loop Leu(75)–Leu(82) which is predicted to encode a Ca(2+) binding site was constructed. Expression of the mutant gene (aprEΔLeu(75)–Leu(82)) produced B. subtilis colonies lacking protease activity. Intrinsic fluorescence analysis revealed s...

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Detalles Bibliográficos
Autores principales: Romero-García, Eliel R., Téllez-Valencia, Alfredo, Trujillo, María F., Sampedro, José G., Nájera, Hugo, Rojo-Domínguez, Arturo, García-Soto, Jesús, Pedraza-Reyes, Mario
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2730478/
https://www.ncbi.nlm.nih.gov/pubmed/19710937
http://dx.doi.org/10.1155/2009/201075

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