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Site-Specific N- and C-Terminal Labeling of a Single Polypeptide Using Sortases of Different Specificity

[Image: see text] The unique reactivity of two sortase enzymes, SrtA(staph) from Staphylococcus aureus and SrtA(strep) from Streptococcus pyogenes, is exploited for site-specific labeling of a single polypeptide with different labels at its N and C termini. SrtA(strep) is used to label the protein’s...

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Detalles Bibliográficos
Autores principales:	Antos, John M., Chew, Guo-Liang, Guimaraes, Carla P., Yoder, Nicholas C., Grotenbreg, Gijsbert M., Popp, Maximilian Wei-Lin, Ploegh, Hidde L.
Formato:	Texto
Lenguaje:	English
Publicado:	American Chemical Society 2009
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2730784/ https://www.ncbi.nlm.nih.gov/pubmed/19610623 http://dx.doi.org/10.1021/ja902681k

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