Simultaneous detection of many T-cell specificities using combinatorial tetramer staining

The direct detection of antigen-specific T cells using tetramers of soluble peptide-major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we des...

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Detalles Bibliográficos
Autores principales: Newell, Evan W., Klein, Lawrence O., Yu, Wong, Davis, Mark M.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731062/
https://www.ncbi.nlm.nih.gov/pubmed/19543286
http://dx.doi.org/10.1038/nmeth.1344
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author Newell, Evan W.
Klein, Lawrence O.
Yu, Wong
Davis, Mark M.
author_facet Newell, Evan W.
Klein, Lawrence O.
Yu, Wong
Davis, Mark M.
author_sort Newell, Evan W.
collection PubMed
description The direct detection of antigen-specific T cells using tetramers of soluble peptide-major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect large numbers (≥ 15) of T cell specificities in a single human blood sample.
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spelling pubmed-27310622010-01-01 Simultaneous detection of many T-cell specificities using combinatorial tetramer staining Newell, Evan W. Klein, Lawrence O. Yu, Wong Davis, Mark M. Nat Methods Article The direct detection of antigen-specific T cells using tetramers of soluble peptide-major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect large numbers (≥ 15) of T cell specificities in a single human blood sample. 2009-06-21 2009-07 /pmc/articles/PMC2731062/ /pubmed/19543286 http://dx.doi.org/10.1038/nmeth.1344 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Newell, Evan W.
Klein, Lawrence O.
Yu, Wong
Davis, Mark M.
Simultaneous detection of many T-cell specificities using combinatorial tetramer staining
title Simultaneous detection of many T-cell specificities using combinatorial tetramer staining
title_full Simultaneous detection of many T-cell specificities using combinatorial tetramer staining
title_fullStr Simultaneous detection of many T-cell specificities using combinatorial tetramer staining
title_full_unstemmed Simultaneous detection of many T-cell specificities using combinatorial tetramer staining
title_short Simultaneous detection of many T-cell specificities using combinatorial tetramer staining
title_sort simultaneous detection of many t-cell specificities using combinatorial tetramer staining
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731062/
https://www.ncbi.nlm.nih.gov/pubmed/19543286
http://dx.doi.org/10.1038/nmeth.1344
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