Cargando…

3D fluorescent in situ hybridization using Arabidopsis leaf cryosections and isolated nuclei

BACKGROUND: Fluorescent hybridization techniques are widely used to study the functional organization of different compartments within the mammalian nucleus. However, few examples of such studies are known in the plant kingdom. Indeed, preservation of nuclei 3D structure, which is required for nucle...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tirichine, Leïla, Andrey, Philippe, Biot, Eric, Maurin, Yves, Gaudin, Valérie
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731090/ https://www.ncbi.nlm.nih.gov/pubmed/19650905 http://dx.doi.org/10.1186/1746-4811-5-11

Ejemplares similares

Statistical Analysis of 3D Images Detects Regular Spatial Distributions of Centromeres and Chromocenters in Animal and Plant Nuclei
por: Andrey, Philippe, et al.
Publicado: (2010)

Fluorescence in situ Localization of Pri-miRNAs in Isolated Arabidopsis thaliana Nuclei
por: Gulanicz, Tomasz, et al.
Publicado: (2023)

Target fluorescence in-situ hybridization (Target FISH) for plasma cell enrichment in myeloma
por: Ma, Edmond S. K., et al.
Publicado: (2016)

Visualization of the impatiens downy mildew pathogen using fluorescence in situ hybridization (FISH)
por: Salgado-Salazar, Catalina, et al.
Publicado: (2018)

Visualization of spatial gene expression in plants by modified RNAscope fluorescent in situ hybridization
por: Solanki, Shyam, et al.
Publicado: (2020)

Protocol: Chromatin immunoprecipitation (ChIP) methodology to investigate histone modifications in two model diatom species
por: Lin, Xin, et al.
Publicado: (2012)

Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain
por: Lauter, Gilbert, et al.
Publicado: (2011)

A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol
por: Huang, Tian, et al.
Publicado: (2023)

FACS-based purification of Arabidopsis microspores, sperm cells and vegetative nuclei
por: Borges, Filipe, et al.
Publicado: (2012)

A novel approach for direct detection of the IGH::CRLF2 gene fusion by fluorescent in situ hybridization
por: González-Arreola, Rosa María, et al.
Publicado: (2023)

A hybrid blob-slice model for accurate and efficient detection of fluorescence labeled nuclei in 3D
por: Santella, Anthony, et al.
Publicado: (2010)

Chlorophyll a fluorescence, under half of the adaptive growth-irradiance, for high-throughput sensing of leaf-water deficit in Arabidopsis thaliana accessions
por: Mishra, Kumud B., et al.
Publicado: (2016)

Cryosectioning Method for Microdissection of Murine Colonic Mucosa
por: Farkas, Attila E., et al.
Publicado: (2015)

Step by Step Golgi-Cox Staining for Cryosection
por: Zhong, Feng, et al.
Publicado: (2019)

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos
por: Ferguson, Jordan L., et al.
Publicado: (2019)

An improved method for preparation of mouse retinal cryosections
por: Li, Ling, et al.
Publicado: (2020)

A modified method for enzymatic isolation of and subsequent wax extraction from Arabidopsis thaliana leaf cuticle
por: Vráblová, Martina, et al.
Publicado: (2020)

Detection of chromosome aberrations in interphase nuclei using fluorescence in situ hybridization technique.
por: Song, H. G., et al.
Publicado: (1993)

One-fits-all pretreatment protocol facilitating Fluorescence In Situ Hybridization on formalin-fixed paraffin-embedded, fresh frozen and cytological slides
por: Richardson, Shivanand O., et al.
Publicado: (2019)

Development of automated brightfield double In Situ hybridization (BDISH) application for HER2 gene and chromosome 17 centromere (CEN 17) for breast carcinomas and an assay performance comparison to manual dual color HER2 fluorescence In Situ hybridization (FISH)
por: Nitta, Hiroaki, et al.
Publicado: (2008)

Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants
por: Ursache, Robertas, et al.
Publicado: (2021)

Two-color fluorescent in situ hybridization in the embryonic zebrafish brain using differential detection systems
por: Lauter, Gilbert, et al.
Publicado: (2011)

Spatial genome organization: contrasting views from chromosome conformation capture and fluorescence in situ hybridization
por: Williamson, Iain, et al.
Publicado: (2014)

The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana
por: Wagner, Lucas, et al.
Publicado: (2017)

Stratification of non-small cell lung cancer patients for therapy with epidermal growth factor receptor inhibitors: the EGFR fluorescence in situ hybridization assay
por: Varella-Garcia, Marileila
Publicado: (2006)

Development of a flow-fluorescence in situ hybridization protocol for the analysis of microbial communities in anaerobic fermentation liquor
por: Nettmann, Edith, et al.
Publicado: (2013)

Investigating residual leukemic cells in acute lymphoblastic leukemia: a practical approach using a streamlined interphase fluorescence in situ hybridization method on cerebrospinal fluid
por: Karapetyan, Knarik, et al.
Publicado: (2023)

Engineering cell-fluorescent ion track hybrid detectors
por: Niklas, Martin, et al.
Publicado: (2013)

A quick protocol for the preparation of mouse retinal cryosections for immunohistochemistry
por: Yang, Jialiang, et al.
Publicado: (2021)

Variable-angle total internal reflection fluorescence microscopy of intact cells of Arabidopsis thaliana
por: Wan, Yinglang, et al.
Publicado: (2011)

Membrane connectivity estimated by digital image analysis of HER2 immunohistochemistry is concordant with visual scoring and fluorescence in situ hybridization results: algorithm evaluation on breast cancer tissue microarrays
por: Laurinaviciene, Aida, et al.
Publicado: (2011)

High-contrast three-dimensional imaging of the Arabidopsis leaf enables the analysis of cell dimensions in the epidermis and mesophyll
por: Wuyts, Nathalie, et al.
Publicado: (2010)

Visualization of plant viral suppressor silencing activity in intact leaf lamina by quantitative fluorescent imaging
por: Stephan, Dirk, et al.
Publicado: (2011)

Rapid fluorescent reporter quantification by leaf disc analysis and its application in plant-virus studies
por: Pasin, Fabio, et al.
Publicado: (2014)

In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections
por: Ellison, Mitchell A., et al.
Publicado: (2016)

A device for single leaf labelling with CO(2) isotopes to study carbon allocation and partitioning in Arabidopsis thaliana
por: Kölling, Katharina, et al.
Publicado: (2013)

Reference genes for quantitative Arabidopsis single molecule RNA fluorescence in situ hybridization
por: Duncan, Susan, et al.
Publicado: (2022)

Chlorophyll fluorescence imaging accurately quantifies freezing damage and cold acclimation responses in Arabidopsis leaves
por: Ehlert, Britta, et al.
Publicado: (2008)

Chlorophyll fluorescence emission can screen cold tolerance of cold acclimated Arabidopsis thaliana accessions
por: Mishra, Anamika, et al.
Publicado: (2014)

Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method
por: Wu, Fu-Hui, et al.
Publicado: (2009)

Cannot write session to /tmp/vufind_sessions/sess_k6vjfnh252b5gpriksfg2ma96l