Antigenic protein modifications in Ehrlichia

To develop effective vaccination strategies againstEhrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed withE. muris were resistant to lethal infection withIxodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming...

Descripción completa

Detalles Bibliográficos
Autores principales: THOMAS, S, THIRUMALAPURA, N, CROSSLEY, E C, ISMAIL, N, WALKER, D H
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2009
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731653/
https://www.ncbi.nlm.nih.gov/pubmed/19493209
http://dx.doi.org/10.1111/j.1365-3024.2009.01099.x
_version_ 1782170959540649984
author THOMAS, S
THIRUMALAPURA, N
CROSSLEY, E C
ISMAIL, N
WALKER, D H
author_facet THOMAS, S
THIRUMALAPURA, N
CROSSLEY, E C
ISMAIL, N
WALKER, D H
author_sort THOMAS, S
collection PubMed
description To develop effective vaccination strategies againstEhrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed withE. muris were resistant to lethal infection withIxodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming withE. muris and later injected with IOE-detected antigenic proteins inE. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both theE. muris and IOE cell lysates with IOE andE. muris-specific polyclonal antibody. Analysis of the total proteins ofE. muris and IOE by two dimensional electrophoresis showed that bothE. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed thatE. muris proteins are more lipoylated and glycosylated than those of IOE.
format Text
id pubmed-2731653
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-27316532010-06-01 Antigenic protein modifications in Ehrlichia THOMAS, S THIRUMALAPURA, N CROSSLEY, E C ISMAIL, N WALKER, D H Parasite Immunol Original Articles To develop effective vaccination strategies againstEhrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed withE. muris were resistant to lethal infection withIxodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming withE. muris and later injected with IOE-detected antigenic proteins inE. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both theE. muris and IOE cell lysates with IOE andE. muris-specific polyclonal antibody. Analysis of the total proteins ofE. muris and IOE by two dimensional electrophoresis showed that bothE. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed thatE. muris proteins are more lipoylated and glycosylated than those of IOE. Blackwell Publishing Ltd 2009-06 /pmc/articles/PMC2731653/ /pubmed/19493209 http://dx.doi.org/10.1111/j.1365-3024.2009.01099.x Text en Journal compilation © 2009 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Original Articles
THOMAS, S
THIRUMALAPURA, N
CROSSLEY, E C
ISMAIL, N
WALKER, D H
Antigenic protein modifications in Ehrlichia
title Antigenic protein modifications in Ehrlichia
title_full Antigenic protein modifications in Ehrlichia
title_fullStr Antigenic protein modifications in Ehrlichia
title_full_unstemmed Antigenic protein modifications in Ehrlichia
title_short Antigenic protein modifications in Ehrlichia
title_sort antigenic protein modifications in ehrlichia
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731653/
https://www.ncbi.nlm.nih.gov/pubmed/19493209
http://dx.doi.org/10.1111/j.1365-3024.2009.01099.x
work_keys_str_mv AT thomass antigenicproteinmodificationsinehrlichia
AT thirumalapuran antigenicproteinmodificationsinehrlichia
AT crossleyec antigenicproteinmodificationsinehrlichia
AT ismailn antigenicproteinmodificationsinehrlichia
AT walkerdh antigenicproteinmodificationsinehrlichia

Ejemplares similares