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Conditional gene silencing of multiple genes with antisense RNAs and generation of a mutator strain of Escherichia coli
In this study, we describe a method of simultaneous conditional gene silencing of up to four genes in Escherichia coli by using antisense RNAs. We used antisense RNAs with paired termini, which carried flanking inverted repeats to create paired double-stranded RNA termini; these RNAs have been prove...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2009
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731896/ https://www.ncbi.nlm.nih.gov/pubmed/19515932 http://dx.doi.org/10.1093/nar/gkp498 |