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Structural characterization of cationic lipid–tRNA complexes

Despite considerable interest and investigations on cationic lipid–DNA complexes, reports on lipid–RNA interaction are very limited. In contrast to lipid–DNA complexes where lipid binding induces partial B to A and B to C conformational changes, lipid–tRNA complexation preserves tRNA folded state. T...

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Autores principales: Marty, Regis, N’soukpoé-Kossi, Christophe N., Charbonneau, David M., Kreplak, Laurent, Tajmir-Riahi, Heidar-Ali
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731917/
https://www.ncbi.nlm.nih.gov/pubmed/19561199
http://dx.doi.org/10.1093/nar/gkp543
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author Marty, Regis
N’soukpoé-Kossi, Christophe N.
Charbonneau, David M.
Kreplak, Laurent
Tajmir-Riahi, Heidar-Ali
author_facet Marty, Regis
N’soukpoé-Kossi, Christophe N.
Charbonneau, David M.
Kreplak, Laurent
Tajmir-Riahi, Heidar-Ali
author_sort Marty, Regis
collection PubMed
description Despite considerable interest and investigations on cationic lipid–DNA complexes, reports on lipid–RNA interaction are very limited. In contrast to lipid–DNA complexes where lipid binding induces partial B to A and B to C conformational changes, lipid–tRNA complexation preserves tRNA folded state. This study is the first attempt to investigate the binding of cationic lipid with transfer RNA and the effect of lipid complexation on tRNA aggregation and condensation. We examine the interaction of tRNA with cholesterol (Chol), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), dioctadecyldimethylammoniumbromide (DDAB) and dioleoylphosphatidylethanolamine (DOPE), at physiological condition, using constant tRNA concentration and various lipid contents. FTIR, UV-visible, CD spectroscopic methods and atomic force microscopy (AFM) were used to analyze lipid binding site, the binding constant and the effects of lipid interaction on tRNA stability, conformation and condensation. Structural analysis showed lipid–tRNA interactions with G–C and A–U base pairs as well as the backbone phosphate group with overall binding constants of K(Chol) = 5.94 (± 0.8) × 10(4) M(–1), K(DDAB) = 8.33 (± 0.90) × 10(5) M(–1), K(DOTAP) = 1.05 (± 0.30) × 10(5) M(–1) and K(DOPE) = 2.75 (± 0.50) × 10(4) M(–1). The order of stability of lipid–tRNA complexation is DDAB > DOTAP > Chol > DOPE. Hydrophobic interactions between lipid aliphatic tails and tRNA were observed. RNA remains in A-family structure, while biopolymer aggregation and condensation occurred at high lipid concentrations.
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spelling pubmed-27319172009-09-10 Structural characterization of cationic lipid–tRNA complexes Marty, Regis N’soukpoé-Kossi, Christophe N. Charbonneau, David M. Kreplak, Laurent Tajmir-Riahi, Heidar-Ali Nucleic Acids Res RNA Despite considerable interest and investigations on cationic lipid–DNA complexes, reports on lipid–RNA interaction are very limited. In contrast to lipid–DNA complexes where lipid binding induces partial B to A and B to C conformational changes, lipid–tRNA complexation preserves tRNA folded state. This study is the first attempt to investigate the binding of cationic lipid with transfer RNA and the effect of lipid complexation on tRNA aggregation and condensation. We examine the interaction of tRNA with cholesterol (Chol), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), dioctadecyldimethylammoniumbromide (DDAB) and dioleoylphosphatidylethanolamine (DOPE), at physiological condition, using constant tRNA concentration and various lipid contents. FTIR, UV-visible, CD spectroscopic methods and atomic force microscopy (AFM) were used to analyze lipid binding site, the binding constant and the effects of lipid interaction on tRNA stability, conformation and condensation. Structural analysis showed lipid–tRNA interactions with G–C and A–U base pairs as well as the backbone phosphate group with overall binding constants of K(Chol) = 5.94 (± 0.8) × 10(4) M(–1), K(DDAB) = 8.33 (± 0.90) × 10(5) M(–1), K(DOTAP) = 1.05 (± 0.30) × 10(5) M(–1) and K(DOPE) = 2.75 (± 0.50) × 10(4) M(–1). The order of stability of lipid–tRNA complexation is DDAB > DOTAP > Chol > DOPE. Hydrophobic interactions between lipid aliphatic tails and tRNA were observed. RNA remains in A-family structure, while biopolymer aggregation and condensation occurred at high lipid concentrations. Oxford University Press 2009-08 2009-06-26 /pmc/articles/PMC2731917/ /pubmed/19561199 http://dx.doi.org/10.1093/nar/gkp543 Text en © 2009 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Marty, Regis
N’soukpoé-Kossi, Christophe N.
Charbonneau, David M.
Kreplak, Laurent
Tajmir-Riahi, Heidar-Ali
Structural characterization of cationic lipid–tRNA complexes
title Structural characterization of cationic lipid–tRNA complexes
title_full Structural characterization of cationic lipid–tRNA complexes
title_fullStr Structural characterization of cationic lipid–tRNA complexes
title_full_unstemmed Structural characterization of cationic lipid–tRNA complexes
title_short Structural characterization of cationic lipid–tRNA complexes
title_sort structural characterization of cationic lipid–trna complexes
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731917/
https://www.ncbi.nlm.nih.gov/pubmed/19561199
http://dx.doi.org/10.1093/nar/gkp543
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