Identification of Comamonas species using 16S rRNA gene sequence

A bacterial strain Bz02 was isolated from a water sample collected from river Gomti at the Indian city of Lucknow. We characterized the strain using 16S rRNA sequence. Phylogenetic analysis showed that the strain formed a monophyletic clade with members of the genus Comamonas. The closest phylogenet...

Descripción completa

Detalles Bibliográficos
Autores principales: Yadav, Vimlesh, Prakash, Satya, Srivastava, Shipra, Verma, Praveen Chandra, Gupta, Vijayta, Basu, Vaishali, Rawat, Anil Kumar
Formato: Texto
Lenguaje:English
Publicado: Biomedical Informatics Publishing Group 2009
Materias:
Hypothesis
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2732032/
https://www.ncbi.nlm.nih.gov/pubmed/19759812
_version_ 1782170999233445888
author Yadav, Vimlesh
Prakash, Satya
Srivastava, Shipra
Verma, Praveen Chandra
Gupta, Vijayta
Basu, Vaishali
Rawat, Anil Kumar
author_facet Yadav, Vimlesh
Prakash, Satya
Srivastava, Shipra
Verma, Praveen Chandra
Gupta, Vijayta
Basu, Vaishali
Rawat, Anil Kumar
author_sort Yadav, Vimlesh
collection PubMed
description A bacterial strain Bz02 was isolated from a water sample collected from river Gomti at the Indian city of Lucknow. We characterized the strain using 16S rRNA sequence. Phylogenetic analysis showed that the strain formed a monophyletic clade with members of the genus Comamonas. The closest phylogenetic relative was Comamonas testosteroni with 95% 16S rRNA gene sequence similarity. It is proposed that the identified strain Bz02 be assigned as the type strain of a species of the genus Comamonas (Comamonas sp Bz02) based on 16S rRNA gene sequence search in Ribosomal Database Project, small subunit rRNA and large subunit rRNA databases together with the phylogenetic tree analysis. The sequence is deposted in GenBank with the accession number FJ211417.
format Text
id pubmed-2732032
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Biomedical Informatics Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-27320322009-09-16 Identification of Comamonas species using 16S rRNA gene sequence Yadav, Vimlesh Prakash, Satya Srivastava, Shipra Verma, Praveen Chandra Gupta, Vijayta Basu, Vaishali Rawat, Anil Kumar Bioinformation Hypothesis A bacterial strain Bz02 was isolated from a water sample collected from river Gomti at the Indian city of Lucknow. We characterized the strain using 16S rRNA sequence. Phylogenetic analysis showed that the strain formed a monophyletic clade with members of the genus Comamonas. The closest phylogenetic relative was Comamonas testosteroni with 95% 16S rRNA gene sequence similarity. It is proposed that the identified strain Bz02 be assigned as the type strain of a species of the genus Comamonas (Comamonas sp Bz02) based on 16S rRNA gene sequence search in Ribosomal Database Project, small subunit rRNA and large subunit rRNA databases together with the phylogenetic tree analysis. The sequence is deposted in GenBank with the accession number FJ211417. Biomedical Informatics Publishing Group 2009-06-13 /pmc/articles/PMC2732032/ /pubmed/19759812 Text en © 2009 Biomedical Informatics Publishing Group This is an open-access article, which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original author and source are credited.
spellingShingle Hypothesis
Yadav, Vimlesh
Prakash, Satya
Srivastava, Shipra
Verma, Praveen Chandra
Gupta, Vijayta
Basu, Vaishali
Rawat, Anil Kumar
Identification of Comamonas species using 16S rRNA gene sequence
title Identification of Comamonas species using 16S rRNA gene sequence
title_full Identification of Comamonas species using 16S rRNA gene sequence
title_fullStr Identification of Comamonas species using 16S rRNA gene sequence
title_full_unstemmed Identification of Comamonas species using 16S rRNA gene sequence
title_short Identification of Comamonas species using 16S rRNA gene sequence
title_sort identification of comamonas species using 16s rrna gene sequence
topic Hypothesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2732032/
https://www.ncbi.nlm.nih.gov/pubmed/19759812
work_keys_str_mv AT yadavvimlesh identificationofcomamonasspeciesusing16srrnagenesequence
AT prakashsatya identificationofcomamonasspeciesusing16srrnagenesequence
AT srivastavashipra identificationofcomamonasspeciesusing16srrnagenesequence
AT vermapraveenchandra identificationofcomamonasspeciesusing16srrnagenesequence
AT guptavijayta identificationofcomamonasspeciesusing16srrnagenesequence
AT basuvaishali identificationofcomamonasspeciesusing16srrnagenesequence
AT rawatanilkumar identificationofcomamonasspeciesusing16srrnagenesequence

Ejemplares similares