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Role of airway lactoperoxidase in scavenging of hydrogen peroxide damage in asthma
Hydrogen peroxide (H(2)O(2)) that is mainly generated by neutrophils and eosinophils in asthma is known to be damaging to the airway and to contribute to airway inflammation. The purpose of the present study was to determine the contribution and the role of lactoperoxidase in scavenging airway hydro...
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Formato: | Texto |
Lenguaje: | English |
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Medknow Publications
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2732085/ https://www.ncbi.nlm.nih.gov/pubmed/19727356 http://dx.doi.org/10.4103/1817-1737.33698 |
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author | Al Obaidi, Amina Hamed Ahmad |
author_facet | Al Obaidi, Amina Hamed Ahmad |
author_sort | Al Obaidi, Amina Hamed Ahmad |
collection | PubMed |
description | Hydrogen peroxide (H(2)O(2)) that is mainly generated by neutrophils and eosinophils in asthma is known to be damaging to the airway and to contribute to airway inflammation. The purpose of the present study was to determine the contribution and the role of lactoperoxidase in scavenging airway hydrogen peroxide, in order to propose a therapeutic approach for asthma. The study was an open clinical trial. Twenty-five nonsmoking asthmatic patients were included in the study. Of them, 16 patients (64%) were male and 9 (36%) were female, with age ranging from 29 to 48 years (45.13 ± 4.6). Of the 25 patients included in the study, only 16 patients completed the study; and they were eligible for analyses. Exhaled breath condensate was collected from all patients at the time of entering the study; and 2, 4 and 8 weeks later. All patients received dapson as a lactoperoxidase inhibitor at a dose of 50 mg daily for 8 weeks. The study was conducted during the period from January 2006 to end of October 2006. H(2)O(2) concentration was determined by an enzymatic assay. Determination of exhaled breath condensate for hydrogen peroxide concentration after 8 weeks of dapson usage indicated an increase (1.05 ± 0.36 μM; 95% CI, 0.89-1.21) as compared to that at baseline (P < 0.0001), 2 weeks (P < 0.001) and 4 weeks (P > 0.05). The increase in hydrogen peroxide concentration in exhaled breath condensate after inhibition of lactoperoxidase by dapson advocates a potential role for lactoperoxidase in scavenging of hydrogen peroxide in asthmatic airway. |
format | Text |
id | pubmed-2732085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Medknow Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-27320852009-09-02 Role of airway lactoperoxidase in scavenging of hydrogen peroxide damage in asthma Al Obaidi, Amina Hamed Ahmad Ann Thorac Med Original Article Hydrogen peroxide (H(2)O(2)) that is mainly generated by neutrophils and eosinophils in asthma is known to be damaging to the airway and to contribute to airway inflammation. The purpose of the present study was to determine the contribution and the role of lactoperoxidase in scavenging airway hydrogen peroxide, in order to propose a therapeutic approach for asthma. The study was an open clinical trial. Twenty-five nonsmoking asthmatic patients were included in the study. Of them, 16 patients (64%) were male and 9 (36%) were female, with age ranging from 29 to 48 years (45.13 ± 4.6). Of the 25 patients included in the study, only 16 patients completed the study; and they were eligible for analyses. Exhaled breath condensate was collected from all patients at the time of entering the study; and 2, 4 and 8 weeks later. All patients received dapson as a lactoperoxidase inhibitor at a dose of 50 mg daily for 8 weeks. The study was conducted during the period from January 2006 to end of October 2006. H(2)O(2) concentration was determined by an enzymatic assay. Determination of exhaled breath condensate for hydrogen peroxide concentration after 8 weeks of dapson usage indicated an increase (1.05 ± 0.36 μM; 95% CI, 0.89-1.21) as compared to that at baseline (P < 0.0001), 2 weeks (P < 0.001) and 4 weeks (P > 0.05). The increase in hydrogen peroxide concentration in exhaled breath condensate after inhibition of lactoperoxidase by dapson advocates a potential role for lactoperoxidase in scavenging of hydrogen peroxide in asthmatic airway. Medknow Publications 2007 /pmc/articles/PMC2732085/ /pubmed/19727356 http://dx.doi.org/10.4103/1817-1737.33698 Text en © Annals of Thoracic Medicine http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Al Obaidi, Amina Hamed Ahmad Role of airway lactoperoxidase in scavenging of hydrogen peroxide damage in asthma |
title | Role of airway lactoperoxidase in scavenging of hydrogen peroxide damage in asthma |
title_full | Role of airway lactoperoxidase in scavenging of hydrogen peroxide damage in asthma |
title_fullStr | Role of airway lactoperoxidase in scavenging of hydrogen peroxide damage in asthma |
title_full_unstemmed | Role of airway lactoperoxidase in scavenging of hydrogen peroxide damage in asthma |
title_short | Role of airway lactoperoxidase in scavenging of hydrogen peroxide damage in asthma |
title_sort | role of airway lactoperoxidase in scavenging of hydrogen peroxide damage in asthma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2732085/ https://www.ncbi.nlm.nih.gov/pubmed/19727356 http://dx.doi.org/10.4103/1817-1737.33698 |
work_keys_str_mv | AT alobaidiaminahamedahmad roleofairwaylactoperoxidaseinscavengingofhydrogenperoxidedamageinasthma |