Vector-based siRNA delivery strategies for high-throughput screening of novel target genes

Application of siRNA in high-throughput fashion is still in its early phase although the principle has been established for three years. In this review, we outline the different vector-based siRNA delivery platforms as well as resources that are becoming available for high-throughput applications, a...

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Detalles Bibliográficos
Autores principales: Chen, Meihong, Du, Quan, Zhang, Hong-Yan, Wahlestedt, Claes, Liang, Zicai
Formato: Texto
Lenguaje:English
Publicado: Library Publishing Media 2005
Materias:
Review Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737198/
https://www.ncbi.nlm.nih.gov/pubmed/19771198
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author Chen, Meihong
Du, Quan
Zhang, Hong-Yan
Wahlestedt, Claes
Liang, Zicai
author_facet Chen, Meihong
Du, Quan
Zhang, Hong-Yan
Wahlestedt, Claes
Liang, Zicai
author_sort Chen, Meihong
collection PubMed
description Application of siRNA in high-throughput fashion is still in its early phase although the principle has been established for three years. In this review, we outline the different vector-based siRNA delivery platforms as well as resources that are becoming available for high-throughput applications, and some initial outcomes of vector siRNA high-throughput screening efforts using vector encoded siRNA. It is expected that further improvement of the siRNA technology and availability of the siRNA resources will help to materialize the potential of siRNA for functional genomics and drug target validation.
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spelling pubmed-27371982009-09-21 Vector-based siRNA delivery strategies for high-throughput screening of novel target genes Chen, Meihong Du, Quan Zhang, Hong-Yan Wahlestedt, Claes Liang, Zicai J RNAi Gene Silencing Review Article Application of siRNA in high-throughput fashion is still in its early phase although the principle has been established for three years. In this review, we outline the different vector-based siRNA delivery platforms as well as resources that are becoming available for high-throughput applications, and some initial outcomes of vector siRNA high-throughput screening efforts using vector encoded siRNA. It is expected that further improvement of the siRNA technology and availability of the siRNA resources will help to materialize the potential of siRNA for functional genomics and drug target validation. Library Publishing Media 2005-07-27 /pmc/articles/PMC2737198/ /pubmed/19771198 Text en © Copyright Meihong Chen et al http://www.libpubmedia.co.uk/RNAiJ/LicenceForUsers.pdf This is an open access article, published under the terms of the Licence for Users available at http://www.libpubmedia.co.uk/RNAiJ/LicenceForUsers.pdf. This licence permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.
spellingShingle Review Article
Chen, Meihong
Du, Quan
Zhang, Hong-Yan
Wahlestedt, Claes
Liang, Zicai
Vector-based siRNA delivery strategies for high-throughput screening of novel target genes
title Vector-based siRNA delivery strategies for high-throughput screening of novel target genes
title_full Vector-based siRNA delivery strategies for high-throughput screening of novel target genes
title_fullStr Vector-based siRNA delivery strategies for high-throughput screening of novel target genes
title_full_unstemmed Vector-based siRNA delivery strategies for high-throughput screening of novel target genes
title_short Vector-based siRNA delivery strategies for high-throughput screening of novel target genes
title_sort vector-based sirna delivery strategies for high-throughput screening of novel target genes
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737198/
https://www.ncbi.nlm.nih.gov/pubmed/19771198
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