Design and application of a versatile expression vector for RNAi in mammalian cells

Here we report a versatile mammalian expression vector called pRIGHT11 for production of small interfering RNA (siRNA) in cells. This vector uses opposing eukaryotic RNA polymerase III promoters U6 and H1 to drive the expression of short siRNA. We have demonstrated the effectiveness of pRIGHT11-gene...

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Detalles Bibliográficos
Autores principales: Lin, Xia, Feng, Xin-Hua
Formato: Texto
Lenguaje:English
Publicado: Library Publishing Media 2005
Materias:
New Methods and Technologies
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737199/
https://www.ncbi.nlm.nih.gov/pubmed/19771203
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author Lin, Xia
Feng, Xin-Hua
author_facet Lin, Xia
Feng, Xin-Hua
author_sort Lin, Xia
collection PubMed
description Here we report a versatile mammalian expression vector called pRIGHT11 for production of small interfering RNA (siRNA) in cells. This vector uses opposing eukaryotic RNA polymerase III promoters U6 and H1 to drive the expression of short siRNA. We have demonstrated the effectiveness of pRIGHT11-generated siRNA in sequence-specific inhibition of transfected reporter genes and endogenous genes. Furthermore, this retrovirus-based vector can carry a random library of siRNA, and thus can be applied to rapid screening of novel genes involved in specific cellular responses.
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spelling pubmed-27371992009-09-21 Design and application of a versatile expression vector for RNAi in mammalian cells Lin, Xia Feng, Xin-Hua J RNAi Gene Silencing New Methods and Technologies Here we report a versatile mammalian expression vector called pRIGHT11 for production of small interfering RNA (siRNA) in cells. This vector uses opposing eukaryotic RNA polymerase III promoters U6 and H1 to drive the expression of short siRNA. We have demonstrated the effectiveness of pRIGHT11-generated siRNA in sequence-specific inhibition of transfected reporter genes and endogenous genes. Furthermore, this retrovirus-based vector can carry a random library of siRNA, and thus can be applied to rapid screening of novel genes involved in specific cellular responses. Library Publishing Media 2005-07-28 /pmc/articles/PMC2737199/ /pubmed/19771203 Text en © Copyright Lin and Feng http://www.libpubmedia.co.uk/RNAiJ/LicenceForUsers.pdf This is an open access article, published under the terms of the Licence for Users available at http://www.libpubmedia.co.uk/RNAiJ/LicenceForUsers.pdf. This licence permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.
spellingShingle New Methods and Technologies
Lin, Xia
Feng, Xin-Hua
Design and application of a versatile expression vector for RNAi in mammalian cells
title Design and application of a versatile expression vector for RNAi in mammalian cells
title_full Design and application of a versatile expression vector for RNAi in mammalian cells
title_fullStr Design and application of a versatile expression vector for RNAi in mammalian cells
title_full_unstemmed Design and application of a versatile expression vector for RNAi in mammalian cells
title_short Design and application of a versatile expression vector for RNAi in mammalian cells
title_sort design and application of a versatile expression vector for rnai in mammalian cells
topic New Methods and Technologies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737199/
https://www.ncbi.nlm.nih.gov/pubmed/19771203
work_keys_str_mv AT linxia designandapplicationofaversatileexpressionvectorforrnaiinmammaliancells
AT fengxinhua designandapplicationofaversatileexpressionvectorforrnaiinmammaliancells

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