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Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway
West Nile virus (WNV) is a member of the Flaviviridae family of vector-borne pathogens. Clinical signs of WNV infection include neurologic symptoms, limb weakness, and encephalitis, which can result in paralysis or death. We report that the WNV-capsid (Cp) by itself induces rapid nuclear condensatio...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Centers for Disease Control and Prevention
2002
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738518/ https://www.ncbi.nlm.nih.gov/pubmed/12498651 http://dx.doi.org/10.3201/eid0812.020224 |
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author | Yang, Joo-Sung Ramanathan, Mathura P. Muthumani, Karuppiah Choo, Andrew Y. Jin, Sung-Ha Yu, Qian-Chun Hwang, Daniel S. Choo, Daniel K. Lee, Mark D. Dang, Kesen Kim, J. Joseph Weiner, David B. |
author_facet | Yang, Joo-Sung Ramanathan, Mathura P. Muthumani, Karuppiah Choo, Andrew Y. Jin, Sung-Ha Yu, Qian-Chun Hwang, Daniel S. Choo, Daniel K. Lee, Mark D. Dang, Kesen Kim, J. Joseph Weiner, David B. |
author_sort | Yang, Joo-Sung |
collection | PubMed |
description | West Nile virus (WNV) is a member of the Flaviviridae family of vector-borne pathogens. Clinical signs of WNV infection include neurologic symptoms, limb weakness, and encephalitis, which can result in paralysis or death. We report that the WNV-capsid (Cp) by itself induces rapid nuclear condensation and cell death in tissue culture. Apoptosis is induced through the mitochondrial pathway resulting in caspase-9 activation and downstream caspase-3 activation. Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. These studies demonstrate that the capsid protein of WNV may be responsible for aspects of viral pathogenesis through induction of the apoptotic cascade. |
format | Text |
id | pubmed-2738518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-27385182009-09-16 Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway Yang, Joo-Sung Ramanathan, Mathura P. Muthumani, Karuppiah Choo, Andrew Y. Jin, Sung-Ha Yu, Qian-Chun Hwang, Daniel S. Choo, Daniel K. Lee, Mark D. Dang, Kesen Kim, J. Joseph Weiner, David B. Emerg Infect Dis Research West Nile virus (WNV) is a member of the Flaviviridae family of vector-borne pathogens. Clinical signs of WNV infection include neurologic symptoms, limb weakness, and encephalitis, which can result in paralysis or death. We report that the WNV-capsid (Cp) by itself induces rapid nuclear condensation and cell death in tissue culture. Apoptosis is induced through the mitochondrial pathway resulting in caspase-9 activation and downstream caspase-3 activation. Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. These studies demonstrate that the capsid protein of WNV may be responsible for aspects of viral pathogenesis through induction of the apoptotic cascade. Centers for Disease Control and Prevention 2002-12 /pmc/articles/PMC2738518/ /pubmed/12498651 http://dx.doi.org/10.3201/eid0812.020224 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
spellingShingle | Research Yang, Joo-Sung Ramanathan, Mathura P. Muthumani, Karuppiah Choo, Andrew Y. Jin, Sung-Ha Yu, Qian-Chun Hwang, Daniel S. Choo, Daniel K. Lee, Mark D. Dang, Kesen Kim, J. Joseph Weiner, David B. Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway |
title | Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway |
title_full | Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway |
title_fullStr | Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway |
title_full_unstemmed | Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway |
title_short | Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway |
title_sort | induction of inflammation by west nile virus capsid through the caspase-9 apoptotic pathway |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738518/ https://www.ncbi.nlm.nih.gov/pubmed/12498651 http://dx.doi.org/10.3201/eid0812.020224 |
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