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Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway

West Nile virus (WNV) is a member of the Flaviviridae family of vector-borne pathogens. Clinical signs of WNV infection include neurologic symptoms, limb weakness, and encephalitis, which can result in paralysis or death. We report that the WNV-capsid (Cp) by itself induces rapid nuclear condensatio...

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Autores principales: Yang, Joo-Sung, Ramanathan, Mathura P., Muthumani, Karuppiah, Choo, Andrew Y., Jin, Sung-Ha, Yu, Qian-Chun, Hwang, Daniel S., Choo, Daniel K., Lee, Mark D., Dang, Kesen, Kim, J. Joseph, Weiner, David B.
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738518/
https://www.ncbi.nlm.nih.gov/pubmed/12498651
http://dx.doi.org/10.3201/eid0812.020224
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author Yang, Joo-Sung
Ramanathan, Mathura P.
Muthumani, Karuppiah
Choo, Andrew Y.
Jin, Sung-Ha
Yu, Qian-Chun
Hwang, Daniel S.
Choo, Daniel K.
Lee, Mark D.
Dang, Kesen
Kim, J. Joseph
Weiner, David B.
author_facet Yang, Joo-Sung
Ramanathan, Mathura P.
Muthumani, Karuppiah
Choo, Andrew Y.
Jin, Sung-Ha
Yu, Qian-Chun
Hwang, Daniel S.
Choo, Daniel K.
Lee, Mark D.
Dang, Kesen
Kim, J. Joseph
Weiner, David B.
author_sort Yang, Joo-Sung
collection PubMed
description West Nile virus (WNV) is a member of the Flaviviridae family of vector-borne pathogens. Clinical signs of WNV infection include neurologic symptoms, limb weakness, and encephalitis, which can result in paralysis or death. We report that the WNV-capsid (Cp) by itself induces rapid nuclear condensation and cell death in tissue culture. Apoptosis is induced through the mitochondrial pathway resulting in caspase-9 activation and downstream caspase-3 activation. Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. These studies demonstrate that the capsid protein of WNV may be responsible for aspects of viral pathogenesis through induction of the apoptotic cascade.
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spelling pubmed-27385182009-09-16 Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway Yang, Joo-Sung Ramanathan, Mathura P. Muthumani, Karuppiah Choo, Andrew Y. Jin, Sung-Ha Yu, Qian-Chun Hwang, Daniel S. Choo, Daniel K. Lee, Mark D. Dang, Kesen Kim, J. Joseph Weiner, David B. Emerg Infect Dis Research West Nile virus (WNV) is a member of the Flaviviridae family of vector-borne pathogens. Clinical signs of WNV infection include neurologic symptoms, limb weakness, and encephalitis, which can result in paralysis or death. We report that the WNV-capsid (Cp) by itself induces rapid nuclear condensation and cell death in tissue culture. Apoptosis is induced through the mitochondrial pathway resulting in caspase-9 activation and downstream caspase-3 activation. Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. These studies demonstrate that the capsid protein of WNV may be responsible for aspects of viral pathogenesis through induction of the apoptotic cascade. Centers for Disease Control and Prevention 2002-12 /pmc/articles/PMC2738518/ /pubmed/12498651 http://dx.doi.org/10.3201/eid0812.020224 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Yang, Joo-Sung
Ramanathan, Mathura P.
Muthumani, Karuppiah
Choo, Andrew Y.
Jin, Sung-Ha
Yu, Qian-Chun
Hwang, Daniel S.
Choo, Daniel K.
Lee, Mark D.
Dang, Kesen
Kim, J. Joseph
Weiner, David B.
Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway
title Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway
title_full Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway
title_fullStr Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway
title_full_unstemmed Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway
title_short Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway
title_sort induction of inflammation by west nile virus capsid through the caspase-9 apoptotic pathway
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738518/
https://www.ncbi.nlm.nih.gov/pubmed/12498651
http://dx.doi.org/10.3201/eid0812.020224
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