Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage

Tb (Tbilisi), the reference Brucellaphage strain, was classified as a member of the Podoviridae family with icosahedral capsids (57 ± 2 nm diameter) and short tails (32 ± 3 nm long). Brucellaphage DNA was double stranded and unmethylated; its molecular size was 34.5 kilobase pairs. Some sequences we...

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Autores principales: Zhu, Cai-Zhong, Xiong, Hong-Yan, Han, Jing, Cui, Bu-Yun, Piao, Dong-Ri, Li, Ya-Fei, Jiang, Hai, Ren, Qian, Ma, Xiang-Yu, Chai, Ya-Ming, Huang, Xia, Zhao, Hong-Yan, Li, Lan-Yu
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738908/
https://www.ncbi.nlm.nih.gov/pubmed/19742121
http://dx.doi.org/10.3390/ijms10072999
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author Zhu, Cai-Zhong
Xiong, Hong-Yan
Han, Jing
Cui, Bu-Yun
Piao, Dong-Ri
Li, Ya-Fei
Jiang, Hai
Ren, Qian
Ma, Xiang-Yu
Chai, Ya-Ming
Huang, Xia
Zhao, Hong-Yan
Li, Lan-Yu
author_facet Zhu, Cai-Zhong
Xiong, Hong-Yan
Han, Jing
Cui, Bu-Yun
Piao, Dong-Ri
Li, Ya-Fei
Jiang, Hai
Ren, Qian
Ma, Xiang-Yu
Chai, Ya-Ming
Huang, Xia
Zhao, Hong-Yan
Li, Lan-Yu
author_sort Zhu, Cai-Zhong
collection PubMed
description Tb (Tbilisi), the reference Brucellaphage strain, was classified as a member of the Podoviridae family with icosahedral capsids (57 ± 2 nm diameter) and short tails (32 ± 3 nm long). Brucellaphage DNA was double stranded and unmethylated; its molecular size was 34.5 kilobase pairs. Some sequences were found through RAPD analysis, TA cloning technology, and structural proteins were observed by using SDS-PAGE. Thus, the results have laid the foundation for the wider use of Brucellaphage’s basic mechanisms and practical applications.
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spelling pubmed-27389082009-09-08 Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage Zhu, Cai-Zhong Xiong, Hong-Yan Han, Jing Cui, Bu-Yun Piao, Dong-Ri Li, Ya-Fei Jiang, Hai Ren, Qian Ma, Xiang-Yu Chai, Ya-Ming Huang, Xia Zhao, Hong-Yan Li, Lan-Yu Int J Mol Sci Article Tb (Tbilisi), the reference Brucellaphage strain, was classified as a member of the Podoviridae family with icosahedral capsids (57 ± 2 nm diameter) and short tails (32 ± 3 nm long). Brucellaphage DNA was double stranded and unmethylated; its molecular size was 34.5 kilobase pairs. Some sequences were found through RAPD analysis, TA cloning technology, and structural proteins were observed by using SDS-PAGE. Thus, the results have laid the foundation for the wider use of Brucellaphage’s basic mechanisms and practical applications. Molecular Diversity Preservation International (MDPI) 2009-07-03 /pmc/articles/PMC2738908/ /pubmed/19742121 http://dx.doi.org/10.3390/ijms10072999 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhu, Cai-Zhong
Xiong, Hong-Yan
Han, Jing
Cui, Bu-Yun
Piao, Dong-Ri
Li, Ya-Fei
Jiang, Hai
Ren, Qian
Ma, Xiang-Yu
Chai, Ya-Ming
Huang, Xia
Zhao, Hong-Yan
Li, Lan-Yu
Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage
title Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage
title_full Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage
title_fullStr Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage
title_full_unstemmed Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage
title_short Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage
title_sort molecular characterization of tb, a new approach for an ancient brucellaphage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738908/
https://www.ncbi.nlm.nih.gov/pubmed/19742121
http://dx.doi.org/10.3390/ijms10072999
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