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Myeloid Cells Contribute to Tumor Lymphangiogenesis
The formation of new blood vessels (angiogenesis) and lymphatic vessels (lymphangiogenesis) promotes tumor outgrowth and metastasis. Previously, it has been demonstrated that bone marrow-derived cells (BMDC) can contribute to tumor angiogenesis. However, the role of BMDC in lymphangiogenesis has lar...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738969/ https://www.ncbi.nlm.nih.gov/pubmed/19759906 http://dx.doi.org/10.1371/journal.pone.0007067 |
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author | Zumsteg, Adrian Baeriswyl, Vanessa Imaizumi, Natsuko Schwendener, Reto Rüegg, Curzio Christofori, Gerhard |
author_facet | Zumsteg, Adrian Baeriswyl, Vanessa Imaizumi, Natsuko Schwendener, Reto Rüegg, Curzio Christofori, Gerhard |
author_sort | Zumsteg, Adrian |
collection | PubMed |
description | The formation of new blood vessels (angiogenesis) and lymphatic vessels (lymphangiogenesis) promotes tumor outgrowth and metastasis. Previously, it has been demonstrated that bone marrow-derived cells (BMDC) can contribute to tumor angiogenesis. However, the role of BMDC in lymphangiogenesis has largely remained elusive. Here, we demonstrate by bone marrow transplantation/reconstitution and genetic lineage-tracing experiments that BMDC integrate into tumor-associated lymphatic vessels in the Rip1Tag2 mouse model of insulinoma and in the TRAMP-C1 prostate cancer transplantation model, and that the integrated BMDC originate from the myelomonocytic lineage. Conversely, pharmacological depletion of tumor-associated macrophages reduces lymphangiogenesis. No cell fusion events are detected by genetic tracing experiments. Rather, the phenotypical conversion of myeloid cells into lymphatic endothelial cells and their integration into lymphatic structures is recapitulated in two in vitro tube formation assays and is dependent on fibroblast growth factor-mediated signaling. Together, the results reveal that myeloid cells can contribute to tumor-associated lymphatic vessels, thus extending the findings on the previously reported role of hematopoietic cells in lymphatic vessel formation. |
format | Text |
id | pubmed-2738969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-27389692009-09-17 Myeloid Cells Contribute to Tumor Lymphangiogenesis Zumsteg, Adrian Baeriswyl, Vanessa Imaizumi, Natsuko Schwendener, Reto Rüegg, Curzio Christofori, Gerhard PLoS One Research Article The formation of new blood vessels (angiogenesis) and lymphatic vessels (lymphangiogenesis) promotes tumor outgrowth and metastasis. Previously, it has been demonstrated that bone marrow-derived cells (BMDC) can contribute to tumor angiogenesis. However, the role of BMDC in lymphangiogenesis has largely remained elusive. Here, we demonstrate by bone marrow transplantation/reconstitution and genetic lineage-tracing experiments that BMDC integrate into tumor-associated lymphatic vessels in the Rip1Tag2 mouse model of insulinoma and in the TRAMP-C1 prostate cancer transplantation model, and that the integrated BMDC originate from the myelomonocytic lineage. Conversely, pharmacological depletion of tumor-associated macrophages reduces lymphangiogenesis. No cell fusion events are detected by genetic tracing experiments. Rather, the phenotypical conversion of myeloid cells into lymphatic endothelial cells and their integration into lymphatic structures is recapitulated in two in vitro tube formation assays and is dependent on fibroblast growth factor-mediated signaling. Together, the results reveal that myeloid cells can contribute to tumor-associated lymphatic vessels, thus extending the findings on the previously reported role of hematopoietic cells in lymphatic vessel formation. Public Library of Science 2009-09-17 /pmc/articles/PMC2738969/ /pubmed/19759906 http://dx.doi.org/10.1371/journal.pone.0007067 Text en Zumsteg et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zumsteg, Adrian Baeriswyl, Vanessa Imaizumi, Natsuko Schwendener, Reto Rüegg, Curzio Christofori, Gerhard Myeloid Cells Contribute to Tumor Lymphangiogenesis |
title | Myeloid Cells Contribute to Tumor Lymphangiogenesis |
title_full | Myeloid Cells Contribute to Tumor Lymphangiogenesis |
title_fullStr | Myeloid Cells Contribute to Tumor Lymphangiogenesis |
title_full_unstemmed | Myeloid Cells Contribute to Tumor Lymphangiogenesis |
title_short | Myeloid Cells Contribute to Tumor Lymphangiogenesis |
title_sort | myeloid cells contribute to tumor lymphangiogenesis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738969/ https://www.ncbi.nlm.nih.gov/pubmed/19759906 http://dx.doi.org/10.1371/journal.pone.0007067 |
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