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Myeloid Cells Contribute to Tumor Lymphangiogenesis

The formation of new blood vessels (angiogenesis) and lymphatic vessels (lymphangiogenesis) promotes tumor outgrowth and metastasis. Previously, it has been demonstrated that bone marrow-derived cells (BMDC) can contribute to tumor angiogenesis. However, the role of BMDC in lymphangiogenesis has lar...

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Autores principales: Zumsteg, Adrian, Baeriswyl, Vanessa, Imaizumi, Natsuko, Schwendener, Reto, Rüegg, Curzio, Christofori, Gerhard
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738969/
https://www.ncbi.nlm.nih.gov/pubmed/19759906
http://dx.doi.org/10.1371/journal.pone.0007067
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author Zumsteg, Adrian
Baeriswyl, Vanessa
Imaizumi, Natsuko
Schwendener, Reto
Rüegg, Curzio
Christofori, Gerhard
author_facet Zumsteg, Adrian
Baeriswyl, Vanessa
Imaizumi, Natsuko
Schwendener, Reto
Rüegg, Curzio
Christofori, Gerhard
author_sort Zumsteg, Adrian
collection PubMed
description The formation of new blood vessels (angiogenesis) and lymphatic vessels (lymphangiogenesis) promotes tumor outgrowth and metastasis. Previously, it has been demonstrated that bone marrow-derived cells (BMDC) can contribute to tumor angiogenesis. However, the role of BMDC in lymphangiogenesis has largely remained elusive. Here, we demonstrate by bone marrow transplantation/reconstitution and genetic lineage-tracing experiments that BMDC integrate into tumor-associated lymphatic vessels in the Rip1Tag2 mouse model of insulinoma and in the TRAMP-C1 prostate cancer transplantation model, and that the integrated BMDC originate from the myelomonocytic lineage. Conversely, pharmacological depletion of tumor-associated macrophages reduces lymphangiogenesis. No cell fusion events are detected by genetic tracing experiments. Rather, the phenotypical conversion of myeloid cells into lymphatic endothelial cells and their integration into lymphatic structures is recapitulated in two in vitro tube formation assays and is dependent on fibroblast growth factor-mediated signaling. Together, the results reveal that myeloid cells can contribute to tumor-associated lymphatic vessels, thus extending the findings on the previously reported role of hematopoietic cells in lymphatic vessel formation.
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spelling pubmed-27389692009-09-17 Myeloid Cells Contribute to Tumor Lymphangiogenesis Zumsteg, Adrian Baeriswyl, Vanessa Imaizumi, Natsuko Schwendener, Reto Rüegg, Curzio Christofori, Gerhard PLoS One Research Article The formation of new blood vessels (angiogenesis) and lymphatic vessels (lymphangiogenesis) promotes tumor outgrowth and metastasis. Previously, it has been demonstrated that bone marrow-derived cells (BMDC) can contribute to tumor angiogenesis. However, the role of BMDC in lymphangiogenesis has largely remained elusive. Here, we demonstrate by bone marrow transplantation/reconstitution and genetic lineage-tracing experiments that BMDC integrate into tumor-associated lymphatic vessels in the Rip1Tag2 mouse model of insulinoma and in the TRAMP-C1 prostate cancer transplantation model, and that the integrated BMDC originate from the myelomonocytic lineage. Conversely, pharmacological depletion of tumor-associated macrophages reduces lymphangiogenesis. No cell fusion events are detected by genetic tracing experiments. Rather, the phenotypical conversion of myeloid cells into lymphatic endothelial cells and their integration into lymphatic structures is recapitulated in two in vitro tube formation assays and is dependent on fibroblast growth factor-mediated signaling. Together, the results reveal that myeloid cells can contribute to tumor-associated lymphatic vessels, thus extending the findings on the previously reported role of hematopoietic cells in lymphatic vessel formation. Public Library of Science 2009-09-17 /pmc/articles/PMC2738969/ /pubmed/19759906 http://dx.doi.org/10.1371/journal.pone.0007067 Text en Zumsteg et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zumsteg, Adrian
Baeriswyl, Vanessa
Imaizumi, Natsuko
Schwendener, Reto
Rüegg, Curzio
Christofori, Gerhard
Myeloid Cells Contribute to Tumor Lymphangiogenesis
title Myeloid Cells Contribute to Tumor Lymphangiogenesis
title_full Myeloid Cells Contribute to Tumor Lymphangiogenesis
title_fullStr Myeloid Cells Contribute to Tumor Lymphangiogenesis
title_full_unstemmed Myeloid Cells Contribute to Tumor Lymphangiogenesis
title_short Myeloid Cells Contribute to Tumor Lymphangiogenesis
title_sort myeloid cells contribute to tumor lymphangiogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738969/
https://www.ncbi.nlm.nih.gov/pubmed/19759906
http://dx.doi.org/10.1371/journal.pone.0007067
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