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Analysis of virulence factors of Helicobacter pylori isolated from a Vietnamese population
BACKGROUND: The incidence of gastric cancer differs among countries in Asia, and it has been suggested that virulence factors associated with Helicobacter pylori are partly responsible. The aim of this study was to investigate several genetic factors regarded as virulence or molecular epidemiologic...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2739534/ https://www.ncbi.nlm.nih.gov/pubmed/19698173 http://dx.doi.org/10.1186/1471-2180-9-175 |
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author | Uchida, Tomohisa Nguyen, Lam Tung Takayama, Akiko Okimoto, Tadayoshi Kodama, Masaaki Murakami, Kazunari Matsuhisa, Takeshi Trinh, Tuan Dung Ta, Long Ho, Dang Quy Dung Hoang, Hoa Hai Kishida, Tetsuko Fujioka, Toshio Moriyama, Masatsugu Yamaoka, Yoshio |
author_facet | Uchida, Tomohisa Nguyen, Lam Tung Takayama, Akiko Okimoto, Tadayoshi Kodama, Masaaki Murakami, Kazunari Matsuhisa, Takeshi Trinh, Tuan Dung Ta, Long Ho, Dang Quy Dung Hoang, Hoa Hai Kishida, Tetsuko Fujioka, Toshio Moriyama, Masatsugu Yamaoka, Yoshio |
author_sort | Uchida, Tomohisa |
collection | PubMed |
description | BACKGROUND: The incidence of gastric cancer differs among countries in Asia, and it has been suggested that virulence factors associated with Helicobacter pylori are partly responsible. The aim of this study was to investigate several genetic factors regarded as virulence or molecular epidemiologic markers in H. pylori isolates from Vietnamese subjects. RESULTS: The cagA, vacA and cag right-end junction genotypes of 103 H. pylori strains from Vietnam (54 from Hanoi and 49 from Ho Chi Minh) were determined by PCR and sequencing. Three types of deletion in the region located upstream of the cagA Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region were identified: the 39-bp deletion type, the 18-bp deletion type, and the no-deletion type. The majority of strains studied (77%; 80/103) had the 18-bp deletion irrespective of geographical location in the country or clinical outcome. All of the 39-bp and 18-bp deletion-type strains possessed the East Asian type cagA repeat region. The type II cag right-end junction genotype was predominant (84%). The vacA m1 genotype was significantly more common in strains isolated in Hanoi, where the incidence of gastric cancer is higher, than in strains from Ho Chi Minh. CONCLUSION: Pre-EPIYA-region typing of the cagA gene could provide a new genetic marker of H. pylori genomic diversity. Our data support the hypothesis that vacA m1 is closely associated with gastric carcinogenesis. |
format | Text |
id | pubmed-2739534 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27395342009-09-09 Analysis of virulence factors of Helicobacter pylori isolated from a Vietnamese population Uchida, Tomohisa Nguyen, Lam Tung Takayama, Akiko Okimoto, Tadayoshi Kodama, Masaaki Murakami, Kazunari Matsuhisa, Takeshi Trinh, Tuan Dung Ta, Long Ho, Dang Quy Dung Hoang, Hoa Hai Kishida, Tetsuko Fujioka, Toshio Moriyama, Masatsugu Yamaoka, Yoshio BMC Microbiol Research article BACKGROUND: The incidence of gastric cancer differs among countries in Asia, and it has been suggested that virulence factors associated with Helicobacter pylori are partly responsible. The aim of this study was to investigate several genetic factors regarded as virulence or molecular epidemiologic markers in H. pylori isolates from Vietnamese subjects. RESULTS: The cagA, vacA and cag right-end junction genotypes of 103 H. pylori strains from Vietnam (54 from Hanoi and 49 from Ho Chi Minh) were determined by PCR and sequencing. Three types of deletion in the region located upstream of the cagA Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region were identified: the 39-bp deletion type, the 18-bp deletion type, and the no-deletion type. The majority of strains studied (77%; 80/103) had the 18-bp deletion irrespective of geographical location in the country or clinical outcome. All of the 39-bp and 18-bp deletion-type strains possessed the East Asian type cagA repeat region. The type II cag right-end junction genotype was predominant (84%). The vacA m1 genotype was significantly more common in strains isolated in Hanoi, where the incidence of gastric cancer is higher, than in strains from Ho Chi Minh. CONCLUSION: Pre-EPIYA-region typing of the cagA gene could provide a new genetic marker of H. pylori genomic diversity. Our data support the hypothesis that vacA m1 is closely associated with gastric carcinogenesis. BioMed Central 2009-08-23 /pmc/articles/PMC2739534/ /pubmed/19698173 http://dx.doi.org/10.1186/1471-2180-9-175 Text en Copyright ©2009 Uchida et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research article Uchida, Tomohisa Nguyen, Lam Tung Takayama, Akiko Okimoto, Tadayoshi Kodama, Masaaki Murakami, Kazunari Matsuhisa, Takeshi Trinh, Tuan Dung Ta, Long Ho, Dang Quy Dung Hoang, Hoa Hai Kishida, Tetsuko Fujioka, Toshio Moriyama, Masatsugu Yamaoka, Yoshio Analysis of virulence factors of Helicobacter pylori isolated from a Vietnamese population |
title | Analysis of virulence factors of Helicobacter pylori isolated from a Vietnamese population |
title_full | Analysis of virulence factors of Helicobacter pylori isolated from a Vietnamese population |
title_fullStr | Analysis of virulence factors of Helicobacter pylori isolated from a Vietnamese population |
title_full_unstemmed | Analysis of virulence factors of Helicobacter pylori isolated from a Vietnamese population |
title_short | Analysis of virulence factors of Helicobacter pylori isolated from a Vietnamese population |
title_sort | analysis of virulence factors of helicobacter pylori isolated from a vietnamese population |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2739534/ https://www.ncbi.nlm.nih.gov/pubmed/19698173 http://dx.doi.org/10.1186/1471-2180-9-175 |
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