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Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities
BACKGROUND: Influenza virus infection causes significant morbidity and mortality and has marked social and economic impacts throughout the world. The influenza surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA), act cooperatively to support efficient influenza A virus replication and p...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2739848/ https://www.ncbi.nlm.nih.gov/pubmed/19678928 http://dx.doi.org/10.1186/1743-422X-6-124 |
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author | Sriwilaijaroen, Nongluk Wilairat, Prapon Hiramatsu, Hiroaki Takahashi, Tadanobu Suzuki, Takashi Ito, Morihiro Ito, Yasuhiko Tashiro, Masato Suzuki, Yasuo |
author_facet | Sriwilaijaroen, Nongluk Wilairat, Prapon Hiramatsu, Hiroaki Takahashi, Tadanobu Suzuki, Takashi Ito, Morihiro Ito, Yasuhiko Tashiro, Masato Suzuki, Yasuo |
author_sort | Sriwilaijaroen, Nongluk |
collection | PubMed |
description | BACKGROUND: Influenza virus infection causes significant morbidity and mortality and has marked social and economic impacts throughout the world. The influenza surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA), act cooperatively to support efficient influenza A virus replication and provide the most important targets for anti-influenza chemotherapy. In this study, povidone-iodine (PVP-I), which has a broad-spectrum microbicidal property, was examined for its inhibitory effects against influenza virus infection in MDCK cells and the mechanisms of PVP-I action on HA and NA were revealed. RESULTS: Results obtained using a novel fluorescence- and chromogenic-based plaque inhibition assay showed that 1.56 mg/ml PVP-I inhibited infections in MDCK cells of human (8 strains) and avian (5 strains) influenza A viruses, including H1N1, H3N2, H5N3 and H9N2, from 23.0–97.5%. A sialidase inhibition assay revealed that PVP-I inhibited N1, N2 and N3 neuraminidases with IC(50 )values of 9.5–212.1 μg/ml by a mixed-type inhibition mechanism. Receptor binding inhibition and hemagglutinin inhibition assays indicated that PVP-I affected viral hemagglutinin rather than host-specific sialic acid receptors. CONCLUSION: Mechanisms of reduction of viral growth in MDCK cells by PVP-I involve blockade of viral attachment to cellular receptors and inhibition of viral release and spread from infected cells. Therefore, PVP-I is useful to prevent infection and limit spread of human and avian influenza viruses. |
format | Text |
id | pubmed-2739848 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27398482009-09-09 Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities Sriwilaijaroen, Nongluk Wilairat, Prapon Hiramatsu, Hiroaki Takahashi, Tadanobu Suzuki, Takashi Ito, Morihiro Ito, Yasuhiko Tashiro, Masato Suzuki, Yasuo Virol J Research BACKGROUND: Influenza virus infection causes significant morbidity and mortality and has marked social and economic impacts throughout the world. The influenza surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA), act cooperatively to support efficient influenza A virus replication and provide the most important targets for anti-influenza chemotherapy. In this study, povidone-iodine (PVP-I), which has a broad-spectrum microbicidal property, was examined for its inhibitory effects against influenza virus infection in MDCK cells and the mechanisms of PVP-I action on HA and NA were revealed. RESULTS: Results obtained using a novel fluorescence- and chromogenic-based plaque inhibition assay showed that 1.56 mg/ml PVP-I inhibited infections in MDCK cells of human (8 strains) and avian (5 strains) influenza A viruses, including H1N1, H3N2, H5N3 and H9N2, from 23.0–97.5%. A sialidase inhibition assay revealed that PVP-I inhibited N1, N2 and N3 neuraminidases with IC(50 )values of 9.5–212.1 μg/ml by a mixed-type inhibition mechanism. Receptor binding inhibition and hemagglutinin inhibition assays indicated that PVP-I affected viral hemagglutinin rather than host-specific sialic acid receptors. CONCLUSION: Mechanisms of reduction of viral growth in MDCK cells by PVP-I involve blockade of viral attachment to cellular receptors and inhibition of viral release and spread from infected cells. Therefore, PVP-I is useful to prevent infection and limit spread of human and avian influenza viruses. BioMed Central 2009-08-13 /pmc/articles/PMC2739848/ /pubmed/19678928 http://dx.doi.org/10.1186/1743-422X-6-124 Text en Copyright © 2009 Sriwilaijaroen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Sriwilaijaroen, Nongluk Wilairat, Prapon Hiramatsu, Hiroaki Takahashi, Tadanobu Suzuki, Takashi Ito, Morihiro Ito, Yasuhiko Tashiro, Masato Suzuki, Yasuo Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities |
title | Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities |
title_full | Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities |
title_fullStr | Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities |
title_full_unstemmed | Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities |
title_short | Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities |
title_sort | mechanisms of the action of povidone-iodine against human and avian influenza a viruses: its effects on hemagglutination and sialidase activities |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2739848/ https://www.ncbi.nlm.nih.gov/pubmed/19678928 http://dx.doi.org/10.1186/1743-422X-6-124 |
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