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Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR
BACKGROUND: Robust designs of PCR-based molecular diagnostic assays rely on the discrimination potential of sequence variants affecting primer-to-template annealing. However, for accurate quantitative PCR (qPCR) assessment of gene expression in populations with gene polymorphisms, the effects of seq...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2741440/ https://www.ncbi.nlm.nih.gov/pubmed/19715565 http://dx.doi.org/10.1186/1472-6750-9-75 |