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Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR

BACKGROUND: Robust designs of PCR-based molecular diagnostic assays rely on the discrimination potential of sequence variants affecting primer-to-template annealing. However, for accurate quantitative PCR (qPCR) assessment of gene expression in populations with gene polymorphisms, the effects of seq...

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Detalles Bibliográficos
Autores principales:	Boyle, Brian, Dallaire, Nancy, MacKay, John
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2741440/ https://www.ncbi.nlm.nih.gov/pubmed/19715565 http://dx.doi.org/10.1186/1472-6750-9-75

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