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Circularly permuted GTPase YqeH binds 30S ribosomal subunit: Implications for its role in ribosome assembly

YqeH, a circularly permuted GTPase, is conserved among bacteria and eukaryotes including humans. It was shown to be essential for the assembly of small ribosomal (30S) subunit in bacteria. However, whether YqeH interacts with 30S ribosome and how it may participate in 30S assembly are not known. Her...

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Autores principales: Anand, Baskaran, Surana, Parag, Bhogaraju, Sagar, Pahari, Sushmita, Prakash, Balaji
Formato: Texto
Lenguaje:English
Publicado: Academic Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2741578/
https://www.ncbi.nlm.nih.gov/pubmed/19540197
http://dx.doi.org/10.1016/j.bbrc.2009.06.078
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author Anand, Baskaran
Surana, Parag
Bhogaraju, Sagar
Pahari, Sushmita
Prakash, Balaji
author_facet Anand, Baskaran
Surana, Parag
Bhogaraju, Sagar
Pahari, Sushmita
Prakash, Balaji
author_sort Anand, Baskaran
collection PubMed
description YqeH, a circularly permuted GTPase, is conserved among bacteria and eukaryotes including humans. It was shown to be essential for the assembly of small ribosomal (30S) subunit in bacteria. However, whether YqeH interacts with 30S ribosome and how it may participate in 30S assembly are not known. Here, using co-sedimentation experiments, we report that YqeH co-associates with 30S ribosome in the GTP-bound form. In order to probe whether YqeH functions as RNA chaperone in 30S assembly, we assayed for strand dissociation and annealing activity. While YqeH does not exhibit these activities, it binds a non-specific single and double-stranded RNA, which unlike the 30S binding is independent of GTP/GDP binding and does not affect intrinsic GTP hydrolysis rates. Further, S5, a ribosomal protein which participates during the initial stages of 30S assembly, was found to promote GTP hydrolysis and RNA binding activities of YqeH.
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spelling pubmed-27415782009-09-17 Circularly permuted GTPase YqeH binds 30S ribosomal subunit: Implications for its role in ribosome assembly Anand, Baskaran Surana, Parag Bhogaraju, Sagar Pahari, Sushmita Prakash, Balaji Biochem Biophys Res Commun Article YqeH, a circularly permuted GTPase, is conserved among bacteria and eukaryotes including humans. It was shown to be essential for the assembly of small ribosomal (30S) subunit in bacteria. However, whether YqeH interacts with 30S ribosome and how it may participate in 30S assembly are not known. Here, using co-sedimentation experiments, we report that YqeH co-associates with 30S ribosome in the GTP-bound form. In order to probe whether YqeH functions as RNA chaperone in 30S assembly, we assayed for strand dissociation and annealing activity. While YqeH does not exhibit these activities, it binds a non-specific single and double-stranded RNA, which unlike the 30S binding is independent of GTP/GDP binding and does not affect intrinsic GTP hydrolysis rates. Further, S5, a ribosomal protein which participates during the initial stages of 30S assembly, was found to promote GTP hydrolysis and RNA binding activities of YqeH. Academic Press 2009-09-04 /pmc/articles/PMC2741578/ /pubmed/19540197 http://dx.doi.org/10.1016/j.bbrc.2009.06.078 Text en © 2009 Elsevier Inc. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Anand, Baskaran
Surana, Parag
Bhogaraju, Sagar
Pahari, Sushmita
Prakash, Balaji
Circularly permuted GTPase YqeH binds 30S ribosomal subunit: Implications for its role in ribosome assembly
title Circularly permuted GTPase YqeH binds 30S ribosomal subunit: Implications for its role in ribosome assembly
title_full Circularly permuted GTPase YqeH binds 30S ribosomal subunit: Implications for its role in ribosome assembly
title_fullStr Circularly permuted GTPase YqeH binds 30S ribosomal subunit: Implications for its role in ribosome assembly
title_full_unstemmed Circularly permuted GTPase YqeH binds 30S ribosomal subunit: Implications for its role in ribosome assembly
title_short Circularly permuted GTPase YqeH binds 30S ribosomal subunit: Implications for its role in ribosome assembly
title_sort circularly permuted gtpase yqeh binds 30s ribosomal subunit: implications for its role in ribosome assembly
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2741578/
https://www.ncbi.nlm.nih.gov/pubmed/19540197
http://dx.doi.org/10.1016/j.bbrc.2009.06.078
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