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Identification of a high incidence region for retroviral vector integration near exon 1 of the LMO2 locus

Therapeutic retroviral vector integration near the oncogene LMO2 is thought to be a cause of leukemia in X-SCID gene therapy trials. However, no published studies have evaluated the frequency of vector integrations near exon 1 of the LMO2 locus. We identified a high incidence region (HIR) of vector...

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Autores principales: Yamada, Koichiro, Tsukahara, Tomonori, Yoshino, Kazuhisa, Kojima, Katsuhiko, Agawa, Hideyuki, Yamashita, Yuki, Amano, Yuji, Hatta, Mariko, Matsuzaki, Yasunori, Kurotori, Naoki, Wakui, Keiko, Fukushima, Yoshimitsu, Osada, Ryosuke, Shiozawa, Tanri, Sakashita, Kazuo, Koike, Kenichi, Kumaki, Satoru, Tanaka, Nobuyuki, Takeshita, Toshikazu
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2742512/
https://www.ncbi.nlm.nih.gov/pubmed/19725963
http://dx.doi.org/10.1186/1742-4690-6-79
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author Yamada, Koichiro
Tsukahara, Tomonori
Yoshino, Kazuhisa
Kojima, Katsuhiko
Agawa, Hideyuki
Yamashita, Yuki
Amano, Yuji
Hatta, Mariko
Matsuzaki, Yasunori
Kurotori, Naoki
Wakui, Keiko
Fukushima, Yoshimitsu
Osada, Ryosuke
Shiozawa, Tanri
Sakashita, Kazuo
Koike, Kenichi
Kumaki, Satoru
Tanaka, Nobuyuki
Takeshita, Toshikazu
author_facet Yamada, Koichiro
Tsukahara, Tomonori
Yoshino, Kazuhisa
Kojima, Katsuhiko
Agawa, Hideyuki
Yamashita, Yuki
Amano, Yuji
Hatta, Mariko
Matsuzaki, Yasunori
Kurotori, Naoki
Wakui, Keiko
Fukushima, Yoshimitsu
Osada, Ryosuke
Shiozawa, Tanri
Sakashita, Kazuo
Koike, Kenichi
Kumaki, Satoru
Tanaka, Nobuyuki
Takeshita, Toshikazu
author_sort Yamada, Koichiro
collection PubMed
description Therapeutic retroviral vector integration near the oncogene LMO2 is thought to be a cause of leukemia in X-SCID gene therapy trials. However, no published studies have evaluated the frequency of vector integrations near exon 1 of the LMO2 locus. We identified a high incidence region (HIR) of vector integration using PCR techniques in the upstream region close to the LMO2 transcription start site in the TPA-Mat T cell line. The integration frequency of the HIR was one per 4.46 × 10(4 )cells. This HIR was also found in Jurkat T cells but was absent from HeLa cells. Furthermore, using human cord blood-derived CD34(+ )cells we identified a HIR in a similar region as the TPA-Mat T cell line. One of the X-linked severe combined immunodeficiency (X-SCID) patients that developed leukemia after gene therapy had a vector integration site in this HIR. Therefore, the descriptions of the location and the integration frequency of the HIR presented here may help us to better understand vector-induced leukemogenesis.
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spelling pubmed-27425122009-09-12 Identification of a high incidence region for retroviral vector integration near exon 1 of the LMO2 locus Yamada, Koichiro Tsukahara, Tomonori Yoshino, Kazuhisa Kojima, Katsuhiko Agawa, Hideyuki Yamashita, Yuki Amano, Yuji Hatta, Mariko Matsuzaki, Yasunori Kurotori, Naoki Wakui, Keiko Fukushima, Yoshimitsu Osada, Ryosuke Shiozawa, Tanri Sakashita, Kazuo Koike, Kenichi Kumaki, Satoru Tanaka, Nobuyuki Takeshita, Toshikazu Retrovirology Short Report Therapeutic retroviral vector integration near the oncogene LMO2 is thought to be a cause of leukemia in X-SCID gene therapy trials. However, no published studies have evaluated the frequency of vector integrations near exon 1 of the LMO2 locus. We identified a high incidence region (HIR) of vector integration using PCR techniques in the upstream region close to the LMO2 transcription start site in the TPA-Mat T cell line. The integration frequency of the HIR was one per 4.46 × 10(4 )cells. This HIR was also found in Jurkat T cells but was absent from HeLa cells. Furthermore, using human cord blood-derived CD34(+ )cells we identified a HIR in a similar region as the TPA-Mat T cell line. One of the X-linked severe combined immunodeficiency (X-SCID) patients that developed leukemia after gene therapy had a vector integration site in this HIR. Therefore, the descriptions of the location and the integration frequency of the HIR presented here may help us to better understand vector-induced leukemogenesis. BioMed Central 2009-09-02 /pmc/articles/PMC2742512/ /pubmed/19725963 http://dx.doi.org/10.1186/1742-4690-6-79 Text en Copyright © 2009 Yamada et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Yamada, Koichiro
Tsukahara, Tomonori
Yoshino, Kazuhisa
Kojima, Katsuhiko
Agawa, Hideyuki
Yamashita, Yuki
Amano, Yuji
Hatta, Mariko
Matsuzaki, Yasunori
Kurotori, Naoki
Wakui, Keiko
Fukushima, Yoshimitsu
Osada, Ryosuke
Shiozawa, Tanri
Sakashita, Kazuo
Koike, Kenichi
Kumaki, Satoru
Tanaka, Nobuyuki
Takeshita, Toshikazu
Identification of a high incidence region for retroviral vector integration near exon 1 of the LMO2 locus
title Identification of a high incidence region for retroviral vector integration near exon 1 of the LMO2 locus
title_full Identification of a high incidence region for retroviral vector integration near exon 1 of the LMO2 locus
title_fullStr Identification of a high incidence region for retroviral vector integration near exon 1 of the LMO2 locus
title_full_unstemmed Identification of a high incidence region for retroviral vector integration near exon 1 of the LMO2 locus
title_short Identification of a high incidence region for retroviral vector integration near exon 1 of the LMO2 locus
title_sort identification of a high incidence region for retroviral vector integration near exon 1 of the lmo2 locus
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2742512/
https://www.ncbi.nlm.nih.gov/pubmed/19725963
http://dx.doi.org/10.1186/1742-4690-6-79
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