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Structural Characterization and Expression Analysis of the SERK/SERL Gene Family in Rice (Oryza sativa)

Somatic embryogenesis (SE) is the developmental restructuring of somatic cells towards the embryogenic pathway and forms the basis of cellular totipotency in angiosperms. With the availability of full-length cDNA sequences from Knowledge-based Oryza Molecular Biological Encylopedia (KOME), we identi...

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Autores principales: Singla, Bhumica, Khurana, Jitendra P., Khurana, Paramjit
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2742738/
https://www.ncbi.nlm.nih.gov/pubmed/19756234
http://dx.doi.org/10.1155/2009/539402
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author Singla, Bhumica
Khurana, Jitendra P.
Khurana, Paramjit
author_facet Singla, Bhumica
Khurana, Jitendra P.
Khurana, Paramjit
author_sort Singla, Bhumica
collection PubMed
description Somatic embryogenesis (SE) is the developmental restructuring of somatic cells towards the embryogenic pathway and forms the basis of cellular totipotency in angiosperms. With the availability of full-length cDNA sequences from Knowledge-based Oryza Molecular Biological Encylopedia (KOME), we identified the leucine-rich repeat receptor-like kinase (LRR-RLK) genes from rice (Oryza sativa), which also encompasses genes involved in regulating somatic embryogenesis. Eight out of eleven of the rice SERK and SERL (SERK-like) genes have the TIGR annotation as (putative) brassinosteroid insensitive 1-associated receptor kinase (precursor). Real-time polymerase chain reaction analysis was undertaken to quantify transcript levels of these 11 genes. Most of these genes were upregulated by brassinosteroids although only a few of these displayed auxin induction. The expression profile of these genes is nearly uniform in the zygotic embryogenic tissue, but the expression pattern is more complex in the somatic embryogenic tissue. It is likely that OsSERKs and OsSERLs may be involved in somatic embryogenesis and also perform a role in morphogenesis and various other plant developmental processes. Functional validation of these somatic embryogenesis receptor-like kinase genes may help in elucidating their precise functions in regulating various facets of plant development.
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spelling pubmed-27427382009-09-15 Structural Characterization and Expression Analysis of the SERK/SERL Gene Family in Rice (Oryza sativa) Singla, Bhumica Khurana, Jitendra P. Khurana, Paramjit Int J Plant Genomics Research Article Somatic embryogenesis (SE) is the developmental restructuring of somatic cells towards the embryogenic pathway and forms the basis of cellular totipotency in angiosperms. With the availability of full-length cDNA sequences from Knowledge-based Oryza Molecular Biological Encylopedia (KOME), we identified the leucine-rich repeat receptor-like kinase (LRR-RLK) genes from rice (Oryza sativa), which also encompasses genes involved in regulating somatic embryogenesis. Eight out of eleven of the rice SERK and SERL (SERK-like) genes have the TIGR annotation as (putative) brassinosteroid insensitive 1-associated receptor kinase (precursor). Real-time polymerase chain reaction analysis was undertaken to quantify transcript levels of these 11 genes. Most of these genes were upregulated by brassinosteroids although only a few of these displayed auxin induction. The expression profile of these genes is nearly uniform in the zygotic embryogenic tissue, but the expression pattern is more complex in the somatic embryogenic tissue. It is likely that OsSERKs and OsSERLs may be involved in somatic embryogenesis and also perform a role in morphogenesis and various other plant developmental processes. Functional validation of these somatic embryogenesis receptor-like kinase genes may help in elucidating their precise functions in regulating various facets of plant development. Hindawi Publishing Corporation 2009 2009-09-13 /pmc/articles/PMC2742738/ /pubmed/19756234 http://dx.doi.org/10.1155/2009/539402 Text en Copyright © 2009 Bhumica Singla et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Singla, Bhumica
Khurana, Jitendra P.
Khurana, Paramjit
Structural Characterization and Expression Analysis of the SERK/SERL Gene Family in Rice (Oryza sativa)
title Structural Characterization and Expression Analysis of the SERK/SERL Gene Family in Rice (Oryza sativa)
title_full Structural Characterization and Expression Analysis of the SERK/SERL Gene Family in Rice (Oryza sativa)
title_fullStr Structural Characterization and Expression Analysis of the SERK/SERL Gene Family in Rice (Oryza sativa)
title_full_unstemmed Structural Characterization and Expression Analysis of the SERK/SERL Gene Family in Rice (Oryza sativa)
title_short Structural Characterization and Expression Analysis of the SERK/SERL Gene Family in Rice (Oryza sativa)
title_sort structural characterization and expression analysis of the serk/serl gene family in rice (oryza sativa)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2742738/
https://www.ncbi.nlm.nih.gov/pubmed/19756234
http://dx.doi.org/10.1155/2009/539402
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