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The Pathogenic Properties of a Novel and Conserved Gene Product, KerV, in Proteobacteria

Identification of novel virulence factors is essential for understanding bacterial pathogenesis and designing antibacterial strategies. In this study, we uncover such a factor, termed KerV, in Proteobacteria. Experiments carried out in a variety of eukaryotic host infection models revealed that the...

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Autores principales: An, Dingding, Apidianakis, Yiorgos, Boechat, Ana Laura, Baldini, Regina L., Goumnerov, Boyan C., Rahme, Laurence G.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2744870/
https://www.ncbi.nlm.nih.gov/pubmed/19779606
http://dx.doi.org/10.1371/journal.pone.0007167
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author An, Dingding
Apidianakis, Yiorgos
Boechat, Ana Laura
Baldini, Regina L.
Goumnerov, Boyan C.
Rahme, Laurence G.
author_facet An, Dingding
Apidianakis, Yiorgos
Boechat, Ana Laura
Baldini, Regina L.
Goumnerov, Boyan C.
Rahme, Laurence G.
author_sort An, Dingding
collection PubMed
description Identification of novel virulence factors is essential for understanding bacterial pathogenesis and designing antibacterial strategies. In this study, we uncover such a factor, termed KerV, in Proteobacteria. Experiments carried out in a variety of eukaryotic host infection models revealed that the virulence of a Pseudomonas aeruginosa kerV null mutant was compromised when it interacted with amoebae, plants, flies, and mice. Bioinformatics analyses indicated that KerV is a hypothetical methyltransferase and is well-conserved across numerous Proteobacteria, including both well-known and emerging pathogens (e.g., virulent Burkholderia, Escherichia, Shigella, Vibrio, Salmonella, Yersinia and Brucella species). Furthermore, among the 197 kerV orthologs analyzed in this study, about 89% reside in a defined genomic neighborhood, which also possesses essential DNA replication and repair genes and detoxification gene. Finally, infection of Drosophila melanogaster with null mutants demonstrated that KerV orthologs are also crucial in Vibrio cholerae and Yersinia pseudotuberculosis pathogenesis. Our findings suggested that KerV has a novel and broad significance as a virulence factor in pathogenic Proteobacteria and it might serve as a new target for antibiotic drug design.
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spelling pubmed-27448702009-09-25 The Pathogenic Properties of a Novel and Conserved Gene Product, KerV, in Proteobacteria An, Dingding Apidianakis, Yiorgos Boechat, Ana Laura Baldini, Regina L. Goumnerov, Boyan C. Rahme, Laurence G. PLoS One Research Article Identification of novel virulence factors is essential for understanding bacterial pathogenesis and designing antibacterial strategies. In this study, we uncover such a factor, termed KerV, in Proteobacteria. Experiments carried out in a variety of eukaryotic host infection models revealed that the virulence of a Pseudomonas aeruginosa kerV null mutant was compromised when it interacted with amoebae, plants, flies, and mice. Bioinformatics analyses indicated that KerV is a hypothetical methyltransferase and is well-conserved across numerous Proteobacteria, including both well-known and emerging pathogens (e.g., virulent Burkholderia, Escherichia, Shigella, Vibrio, Salmonella, Yersinia and Brucella species). Furthermore, among the 197 kerV orthologs analyzed in this study, about 89% reside in a defined genomic neighborhood, which also possesses essential DNA replication and repair genes and detoxification gene. Finally, infection of Drosophila melanogaster with null mutants demonstrated that KerV orthologs are also crucial in Vibrio cholerae and Yersinia pseudotuberculosis pathogenesis. Our findings suggested that KerV has a novel and broad significance as a virulence factor in pathogenic Proteobacteria and it might serve as a new target for antibiotic drug design. Public Library of Science 2009-09-25 /pmc/articles/PMC2744870/ /pubmed/19779606 http://dx.doi.org/10.1371/journal.pone.0007167 Text en An et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
An, Dingding
Apidianakis, Yiorgos
Boechat, Ana Laura
Baldini, Regina L.
Goumnerov, Boyan C.
Rahme, Laurence G.
The Pathogenic Properties of a Novel and Conserved Gene Product, KerV, in Proteobacteria
title The Pathogenic Properties of a Novel and Conserved Gene Product, KerV, in Proteobacteria
title_full The Pathogenic Properties of a Novel and Conserved Gene Product, KerV, in Proteobacteria
title_fullStr The Pathogenic Properties of a Novel and Conserved Gene Product, KerV, in Proteobacteria
title_full_unstemmed The Pathogenic Properties of a Novel and Conserved Gene Product, KerV, in Proteobacteria
title_short The Pathogenic Properties of a Novel and Conserved Gene Product, KerV, in Proteobacteria
title_sort pathogenic properties of a novel and conserved gene product, kerv, in proteobacteria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2744870/
https://www.ncbi.nlm.nih.gov/pubmed/19779606
http://dx.doi.org/10.1371/journal.pone.0007167
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