Protein versus DNA as a marker for peripheral blood mononuclear cell counting

Quantitative analysis of intracellular analytes requires an accurate and precise assay not only for the quantitation of the analytes, but also for the quantitation of the number of cells in which they were determined. In this technical note we compare protein and DNA as markers for the number of per...

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Detalles Bibliográficos
Autores principales: Jansen, Robert S., Rosing, Hilde, Schellens, Jan H. M., Beijnen, Jos H.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2745619/
https://www.ncbi.nlm.nih.gov/pubmed/19685233
http://dx.doi.org/10.1007/s00216-009-3022-3
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author Jansen, Robert S.
Rosing, Hilde
Schellens, Jan H. M.
Beijnen, Jos H.
author_facet Jansen, Robert S.
Rosing, Hilde
Schellens, Jan H. M.
Beijnen, Jos H.
author_sort Jansen, Robert S.
collection PubMed
description Quantitative analysis of intracellular analytes requires an accurate and precise assay not only for the quantitation of the analytes, but also for the quantitation of the number of cells in which they were determined. In this technical note we compare protein and DNA as markers for the number of peripheral blood mononuclear cells (PBMCs) isolated from whole blood. The protein content of samples was highly influenced by red blood cell contamination and was, therefore, a less suitable marker. The DNA-based method was unaffected by red blood cell contamination and was finally validated over a range from 10 × 10(6) to 300 × 10(6) PBMCs/mL. [Figure: see text]
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spelling pubmed-27456192009-09-17 Protein versus DNA as a marker for peripheral blood mononuclear cell counting Jansen, Robert S. Rosing, Hilde Schellens, Jan H. M. Beijnen, Jos H. Anal Bioanal Chem Technical Note Quantitative analysis of intracellular analytes requires an accurate and precise assay not only for the quantitation of the analytes, but also for the quantitation of the number of cells in which they were determined. In this technical note we compare protein and DNA as markers for the number of peripheral blood mononuclear cells (PBMCs) isolated from whole blood. The protein content of samples was highly influenced by red blood cell contamination and was, therefore, a less suitable marker. The DNA-based method was unaffected by red blood cell contamination and was finally validated over a range from 10 × 10(6) to 300 × 10(6) PBMCs/mL. [Figure: see text] Springer-Verlag 2009-08-16 2009 /pmc/articles/PMC2745619/ /pubmed/19685233 http://dx.doi.org/10.1007/s00216-009-3022-3 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Technical Note
Jansen, Robert S.
Rosing, Hilde
Schellens, Jan H. M.
Beijnen, Jos H.
Protein versus DNA as a marker for peripheral blood mononuclear cell counting
title Protein versus DNA as a marker for peripheral blood mononuclear cell counting
title_full Protein versus DNA as a marker for peripheral blood mononuclear cell counting
title_fullStr Protein versus DNA as a marker for peripheral blood mononuclear cell counting
title_full_unstemmed Protein versus DNA as a marker for peripheral blood mononuclear cell counting
title_short Protein versus DNA as a marker for peripheral blood mononuclear cell counting
title_sort protein versus dna as a marker for peripheral blood mononuclear cell counting
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2745619/
https://www.ncbi.nlm.nih.gov/pubmed/19685233
http://dx.doi.org/10.1007/s00216-009-3022-3
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