A bright and photostable photoconvertible fluorescent protein for fusion tags

Photoconvertible fluorescent proteins offer significant potential as tools for investigating dynamic processes in living cells and for emerging super-resolution microscopy techniques. Unfortunately, most probes in this class are hampered by oligomerization, small photon budgets, or poor photostability. Here we report an EosFP variant that functions well in a broad range of protein fusions for dynamic investigations, exhibits high photostability, and preserves the superior ~10-nm localization precision of its parent.