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Optimized Quantification of Fragmented, Free Circulating DNA in Human Blood Plasma Using a Calibrated Duplex Real-Time PCR

BACKGROUND: Duplex real-time PCR assays have been widely used to determine amounts and concentrations of free circulating DNA in human blood plasma samples. Circulatory plasma DNA is highly fragmented and hence a PCR-based determination of DNA concentration may be affected by the limited availabilit...

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Detalles Bibliográficos
Autores principales:	Horlitz, Martin, Lucas, Annabelle, Sprenger-Haussels, Markus
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2009
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2746311/ https://www.ncbi.nlm.nih.gov/pubmed/19784371 http://dx.doi.org/10.1371/journal.pone.0007207

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