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Both Conventional and Interferon Killer Dendritic Cells Have Antigen-Presenting Capacity during Influenza Virus Infection

Natural killer cells are innate effector cells known for their potential to produce interferon-γ and kill tumour and virus-infected cells. Recently, B220(+)CD11c(int)NK1.1(+) NK cells were found to also have antigen-presenting capacity like dendritic cells (DC), hence their name interferon-producing...

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Autores principales: GeurtsvanKessel, Corine H., Bergen, Ingrid M., Muskens, Femke, Boon, Louis, Hoogsteden, Henk C., Osterhaus, Albert D. M. E., Rimmelzwaan, Guus F., Lambrecht, Bart N.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747012/
https://www.ncbi.nlm.nih.gov/pubmed/19784375
http://dx.doi.org/10.1371/journal.pone.0007187
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author GeurtsvanKessel, Corine H.
Bergen, Ingrid M.
Muskens, Femke
Boon, Louis
Hoogsteden, Henk C.
Osterhaus, Albert D. M. E.
Rimmelzwaan, Guus F.
Lambrecht, Bart N.
author_facet GeurtsvanKessel, Corine H.
Bergen, Ingrid M.
Muskens, Femke
Boon, Louis
Hoogsteden, Henk C.
Osterhaus, Albert D. M. E.
Rimmelzwaan, Guus F.
Lambrecht, Bart N.
author_sort GeurtsvanKessel, Corine H.
collection PubMed
description Natural killer cells are innate effector cells known for their potential to produce interferon-γ and kill tumour and virus-infected cells. Recently, B220(+)CD11c(int)NK1.1(+) NK cells were found to also have antigen-presenting capacity like dendritic cells (DC), hence their name interferon-producing killer DC (IKDC). Shortly after discovery, it has already been questioned if IKDC really represent a separate subset of NK cells or merely represent a state of activation. Despite similarities with DCs, in vivo evidence that they behave as bona fide APCs is lacking. Here, using a model of influenza infection, we found recruitment of both conventional B220(−) NK cells and IKDCs to the lung. To study antigen-presenting capacity of NK cell subsets and compare it to cDCs, all cell subsets were sorted from lungs of infected mice and co-cultured ex vivo with antigen specific T cells. Both IKDCs and conventional NK cells as well as cDCs presented virus-encoded antigen to CD8 T cells, whereas only cDCs presented to CD4 T cells. The absence of CD4 responses was predominantly due to a deficiency in MHCII processing, as preprocessed peptide antigen was presented equally well by cDCs and IKDCs. In vivo, the depletion of NK1.1-positive NK cells and IKDCs reduced the expansion of viral nucleoprotein-specific CD8 T cells in the lung and spleen, but did finally not affect viral clearance from the lung. In conclusion, we found evidence for APC function of lung NK cells during influenza infection, but this is a feature not exclusive to the IKDC subset.
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spelling pubmed-27470122009-09-28 Both Conventional and Interferon Killer Dendritic Cells Have Antigen-Presenting Capacity during Influenza Virus Infection GeurtsvanKessel, Corine H. Bergen, Ingrid M. Muskens, Femke Boon, Louis Hoogsteden, Henk C. Osterhaus, Albert D. M. E. Rimmelzwaan, Guus F. Lambrecht, Bart N. PLoS One Research Article Natural killer cells are innate effector cells known for their potential to produce interferon-γ and kill tumour and virus-infected cells. Recently, B220(+)CD11c(int)NK1.1(+) NK cells were found to also have antigen-presenting capacity like dendritic cells (DC), hence their name interferon-producing killer DC (IKDC). Shortly after discovery, it has already been questioned if IKDC really represent a separate subset of NK cells or merely represent a state of activation. Despite similarities with DCs, in vivo evidence that they behave as bona fide APCs is lacking. Here, using a model of influenza infection, we found recruitment of both conventional B220(−) NK cells and IKDCs to the lung. To study antigen-presenting capacity of NK cell subsets and compare it to cDCs, all cell subsets were sorted from lungs of infected mice and co-cultured ex vivo with antigen specific T cells. Both IKDCs and conventional NK cells as well as cDCs presented virus-encoded antigen to CD8 T cells, whereas only cDCs presented to CD4 T cells. The absence of CD4 responses was predominantly due to a deficiency in MHCII processing, as preprocessed peptide antigen was presented equally well by cDCs and IKDCs. In vivo, the depletion of NK1.1-positive NK cells and IKDCs reduced the expansion of viral nucleoprotein-specific CD8 T cells in the lung and spleen, but did finally not affect viral clearance from the lung. In conclusion, we found evidence for APC function of lung NK cells during influenza infection, but this is a feature not exclusive to the IKDC subset. Public Library of Science 2009-09-28 /pmc/articles/PMC2747012/ /pubmed/19784375 http://dx.doi.org/10.1371/journal.pone.0007187 Text en GeurtsvanKessel et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
GeurtsvanKessel, Corine H.
Bergen, Ingrid M.
Muskens, Femke
Boon, Louis
Hoogsteden, Henk C.
Osterhaus, Albert D. M. E.
Rimmelzwaan, Guus F.
Lambrecht, Bart N.
Both Conventional and Interferon Killer Dendritic Cells Have Antigen-Presenting Capacity during Influenza Virus Infection
title Both Conventional and Interferon Killer Dendritic Cells Have Antigen-Presenting Capacity during Influenza Virus Infection
title_full Both Conventional and Interferon Killer Dendritic Cells Have Antigen-Presenting Capacity during Influenza Virus Infection
title_fullStr Both Conventional and Interferon Killer Dendritic Cells Have Antigen-Presenting Capacity during Influenza Virus Infection
title_full_unstemmed Both Conventional and Interferon Killer Dendritic Cells Have Antigen-Presenting Capacity during Influenza Virus Infection
title_short Both Conventional and Interferon Killer Dendritic Cells Have Antigen-Presenting Capacity during Influenza Virus Infection
title_sort both conventional and interferon killer dendritic cells have antigen-presenting capacity during influenza virus infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747012/
https://www.ncbi.nlm.nih.gov/pubmed/19784375
http://dx.doi.org/10.1371/journal.pone.0007187
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