Intercellular transfer to signaling endosomes regulates an ex vivo bone marrow niche

Hematopoietic stem-progenitor cells (HSPCs) reside in the bone marrow niche, where interactions with osteoblasts provide essential cues for their proliferation and survival. Here, we use live cell imaging to characterize both the site of contact between osteoblasts and hematopoietic progenitor cells (HPCs) and events at this site that result in downstream signaling responses important for niche maintenance. HPCs made prolonged contact with the osteoblast surface via a specialized membrane domain enriched in prominin 1, CD63 and rhodamine PE. At the contact site, portions of the specialized domain containing these molecules were taken up by the osteoblast and internalized into SARA-positive signaling endosomes. This caused osteoblasts to downregulate Smad signaling and increase production of stromal-derived factor-1 (SDF-1), a chemokine responsible for HSPC homing to bone marrow. These findings identify a mechanism involving intercellular transfer to signaling endosomes for targeted regulation of signaling and remodeling events within an ex vivo osteoblastic niche.