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Transmission of Specific Genotype Streptomycin Resistant Strains of Mycobacterium tuberculosis in the Tokyo Metropolitan Area in Japan

BACKGROUND: From 2003 through to 2004, an outbreak of tuberculosis was identified at a university campus in Yokohama City, located in the southern part of the Tokyo Metropolitan Area (TMA). All Mycobacterium tuberculosis (M. tuberculosis) strains detected with regards to this outbreak turned out to...

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Autores principales: Ohkado, Akihiro, Murase, Yoshiro, Mori, Masaaki, Hasegawa, Naoki, Otsuka, Goro, Nagamine, Michiko, Maeda, Hideo, Uchimura, Kazuhiro, Ohmori, Masako, Yamada, Norio, Maeda, Shinji, Kato, Seiya, Mori, Toru, Ishikawa, Nobukatsu
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2749854/
https://www.ncbi.nlm.nih.gov/pubmed/19709409
http://dx.doi.org/10.1186/1471-2334-9-138
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author Ohkado, Akihiro
Murase, Yoshiro
Mori, Masaaki
Hasegawa, Naoki
Otsuka, Goro
Nagamine, Michiko
Maeda, Hideo
Uchimura, Kazuhiro
Ohmori, Masako
Yamada, Norio
Maeda, Shinji
Kato, Seiya
Mori, Toru
Ishikawa, Nobukatsu
author_facet Ohkado, Akihiro
Murase, Yoshiro
Mori, Masaaki
Hasegawa, Naoki
Otsuka, Goro
Nagamine, Michiko
Maeda, Hideo
Uchimura, Kazuhiro
Ohmori, Masako
Yamada, Norio
Maeda, Shinji
Kato, Seiya
Mori, Toru
Ishikawa, Nobukatsu
author_sort Ohkado, Akihiro
collection PubMed
description BACKGROUND: From 2003 through to 2004, an outbreak of tuberculosis was identified at a university campus in Yokohama City, located in the southern part of the Tokyo Metropolitan Area (TMA). All Mycobacterium tuberculosis (M. tuberculosis) strains detected with regards to this outbreak turned out to be Streptomycin resistant with matched patterns of 14 IS6110 bands of Restriction Fragment Length Polymorphism (RFLP). The M. tuberculosis bacilli, which had the matched IS6110 band patterns with resistance to Streptomycin to those of bacilli isolated in the outbreak, were also concurrently detected through either the population-based or the hospital-based DNA fingerprinting surveillance of M. tuberculosis either in Shinjuku City or in Kawasaki City respectively. The aim of the present study is to describe the spread of the specific genotype strains of M. tuberculosis in the TMA as observed in the above incident, and to identify the possible transmission routes of the strains among people living in urban settings in Japan. METHODS: We applied Variable Numbers of Tandem Repeats (VNTR) analysis to all M. tuberculosis isolates which were resistant to Streptomycin with a matched IS6110-RFLP band pattern (M-strains). They were isolated either from cases related to the tuberculosis outbreak that happened at a university, or through DNA fingerprinting surveillance of M. tuberculosis both in Shinjuku City and in Kawasaki City. For VNTR analysis, 12MIRU loci, 4ETR loci, seven loci by Supply, four loci by Murase (QUB15, Mtub24, VNTR2372, VNTR3336) were selected. RESULTS: Out of a total of 664 isolates collected during the study period, 46 isolates (6.9%) were identified as M-strains. There was a tendency that there was a higher proportion of those patients whose isolates belonged to M4-substrains, with four copies of tandem repeat at the ETR-C locus, to have visited some of the internet-cafés in the TMA than those whose isolates belonged to M5-substrains, with five copies at the ETR-C locus, although statistically not significant (38.1% vs. 10.0%, Exact p = 0.150). CONCLUSION: Although firm conclusions could not be reached through the present study, it suggested that we have to take into consideration that tuberculosis can be transmitted in congregated facilities like internet cafés where tuberculosis high-risk people and general people share common spaces.
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spelling pubmed-27498542009-09-24 Transmission of Specific Genotype Streptomycin Resistant Strains of Mycobacterium tuberculosis in the Tokyo Metropolitan Area in Japan Ohkado, Akihiro Murase, Yoshiro Mori, Masaaki Hasegawa, Naoki Otsuka, Goro Nagamine, Michiko Maeda, Hideo Uchimura, Kazuhiro Ohmori, Masako Yamada, Norio Maeda, Shinji Kato, Seiya Mori, Toru Ishikawa, Nobukatsu BMC Infect Dis Research Article BACKGROUND: From 2003 through to 2004, an outbreak of tuberculosis was identified at a university campus in Yokohama City, located in the southern part of the Tokyo Metropolitan Area (TMA). All Mycobacterium tuberculosis (M. tuberculosis) strains detected with regards to this outbreak turned out to be Streptomycin resistant with matched patterns of 14 IS6110 bands of Restriction Fragment Length Polymorphism (RFLP). The M. tuberculosis bacilli, which had the matched IS6110 band patterns with resistance to Streptomycin to those of bacilli isolated in the outbreak, were also concurrently detected through either the population-based or the hospital-based DNA fingerprinting surveillance of M. tuberculosis either in Shinjuku City or in Kawasaki City respectively. The aim of the present study is to describe the spread of the specific genotype strains of M. tuberculosis in the TMA as observed in the above incident, and to identify the possible transmission routes of the strains among people living in urban settings in Japan. METHODS: We applied Variable Numbers of Tandem Repeats (VNTR) analysis to all M. tuberculosis isolates which were resistant to Streptomycin with a matched IS6110-RFLP band pattern (M-strains). They were isolated either from cases related to the tuberculosis outbreak that happened at a university, or through DNA fingerprinting surveillance of M. tuberculosis both in Shinjuku City and in Kawasaki City. For VNTR analysis, 12MIRU loci, 4ETR loci, seven loci by Supply, four loci by Murase (QUB15, Mtub24, VNTR2372, VNTR3336) were selected. RESULTS: Out of a total of 664 isolates collected during the study period, 46 isolates (6.9%) were identified as M-strains. There was a tendency that there was a higher proportion of those patients whose isolates belonged to M4-substrains, with four copies of tandem repeat at the ETR-C locus, to have visited some of the internet-cafés in the TMA than those whose isolates belonged to M5-substrains, with five copies at the ETR-C locus, although statistically not significant (38.1% vs. 10.0%, Exact p = 0.150). CONCLUSION: Although firm conclusions could not be reached through the present study, it suggested that we have to take into consideration that tuberculosis can be transmitted in congregated facilities like internet cafés where tuberculosis high-risk people and general people share common spaces. BioMed Central 2009-08-26 /pmc/articles/PMC2749854/ /pubmed/19709409 http://dx.doi.org/10.1186/1471-2334-9-138 Text en Copyright ©2009 Ohkado et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ohkado, Akihiro
Murase, Yoshiro
Mori, Masaaki
Hasegawa, Naoki
Otsuka, Goro
Nagamine, Michiko
Maeda, Hideo
Uchimura, Kazuhiro
Ohmori, Masako
Yamada, Norio
Maeda, Shinji
Kato, Seiya
Mori, Toru
Ishikawa, Nobukatsu
Transmission of Specific Genotype Streptomycin Resistant Strains of Mycobacterium tuberculosis in the Tokyo Metropolitan Area in Japan
title Transmission of Specific Genotype Streptomycin Resistant Strains of Mycobacterium tuberculosis in the Tokyo Metropolitan Area in Japan
title_full Transmission of Specific Genotype Streptomycin Resistant Strains of Mycobacterium tuberculosis in the Tokyo Metropolitan Area in Japan
title_fullStr Transmission of Specific Genotype Streptomycin Resistant Strains of Mycobacterium tuberculosis in the Tokyo Metropolitan Area in Japan
title_full_unstemmed Transmission of Specific Genotype Streptomycin Resistant Strains of Mycobacterium tuberculosis in the Tokyo Metropolitan Area in Japan
title_short Transmission of Specific Genotype Streptomycin Resistant Strains of Mycobacterium tuberculosis in the Tokyo Metropolitan Area in Japan
title_sort transmission of specific genotype streptomycin resistant strains of mycobacterium tuberculosis in the tokyo metropolitan area in japan
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2749854/
https://www.ncbi.nlm.nih.gov/pubmed/19709409
http://dx.doi.org/10.1186/1471-2334-9-138
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