SP1 enhances Zbtb7A gene expression via direct binding to GC box in HePG2 cells

BACKGROUND: Zbtb7A is a proto-oncogenic transcriptional regulator that plays an important role in adipogenesis, osteogenesis and oncogenesis, but little is known about the regulation of Zbtb7A gene expression which is of importance in the function uncovering of this gene. FINDING: Here, a 5'-fl...

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Autores principales: Zu, Xuyu, Yu, Lingling, Sun, Qinsheng, Liu, Feng, Wang, Jue, Xie, Zhenhua, Wang, Ying, Xu, Wei, Jiang, Yuyang
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2749864/
https://www.ncbi.nlm.nih.gov/pubmed/19723341
http://dx.doi.org/10.1186/1756-0500-2-175
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author Zu, Xuyu
Yu, Lingling
Sun, Qinsheng
Liu, Feng
Wang, Jue
Xie, Zhenhua
Wang, Ying
Xu, Wei
Jiang, Yuyang
author_facet Zu, Xuyu
Yu, Lingling
Sun, Qinsheng
Liu, Feng
Wang, Jue
Xie, Zhenhua
Wang, Ying
Xu, Wei
Jiang, Yuyang
author_sort Zu, Xuyu
collection PubMed
description BACKGROUND: Zbtb7A is a proto-oncogenic transcriptional regulator that plays an important role in adipogenesis, osteogenesis and oncogenesis, but little is known about the regulation of Zbtb7A gene expression which is of importance in the function uncovering of this gene. FINDING: Here, a 5'-flanking region of the human Zbtb7A gene was cloned and characterized. It was found that the GC box within Zbtb7A promoter is necessary for the promoter activity. Furthermore, we identified that Sp1 acts as an activator in the regulation of Zbtb7A promoter activity and the physical interaction between Sp1 and GC box is responsible for the activation of Zbtb7A gene promoter. CONCLUSION: Our results confirmed that Sp1 upregulates Zbtb7A gene expression via direct binding to GC box within the promoter.
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spelling pubmed-27498642009-09-24 SP1 enhances Zbtb7A gene expression via direct binding to GC box in HePG2 cells Zu, Xuyu Yu, Lingling Sun, Qinsheng Liu, Feng Wang, Jue Xie, Zhenhua Wang, Ying Xu, Wei Jiang, Yuyang BMC Res Notes Short Report BACKGROUND: Zbtb7A is a proto-oncogenic transcriptional regulator that plays an important role in adipogenesis, osteogenesis and oncogenesis, but little is known about the regulation of Zbtb7A gene expression which is of importance in the function uncovering of this gene. FINDING: Here, a 5'-flanking region of the human Zbtb7A gene was cloned and characterized. It was found that the GC box within Zbtb7A promoter is necessary for the promoter activity. Furthermore, we identified that Sp1 acts as an activator in the regulation of Zbtb7A promoter activity and the physical interaction between Sp1 and GC box is responsible for the activation of Zbtb7A gene promoter. CONCLUSION: Our results confirmed that Sp1 upregulates Zbtb7A gene expression via direct binding to GC box within the promoter. BioMed Central 2009-09-02 /pmc/articles/PMC2749864/ /pubmed/19723341 http://dx.doi.org/10.1186/1756-0500-2-175 Text en Copyright © 2009 Xu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Zu, Xuyu
Yu, Lingling
Sun, Qinsheng
Liu, Feng
Wang, Jue
Xie, Zhenhua
Wang, Ying
Xu, Wei
Jiang, Yuyang
SP1 enhances Zbtb7A gene expression via direct binding to GC box in HePG2 cells
title SP1 enhances Zbtb7A gene expression via direct binding to GC box in HePG2 cells
title_full SP1 enhances Zbtb7A gene expression via direct binding to GC box in HePG2 cells
title_fullStr SP1 enhances Zbtb7A gene expression via direct binding to GC box in HePG2 cells
title_full_unstemmed SP1 enhances Zbtb7A gene expression via direct binding to GC box in HePG2 cells
title_short SP1 enhances Zbtb7A gene expression via direct binding to GC box in HePG2 cells
title_sort sp1 enhances zbtb7a gene expression via direct binding to gc box in hepg2 cells
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2749864/
https://www.ncbi.nlm.nih.gov/pubmed/19723341
http://dx.doi.org/10.1186/1756-0500-2-175
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