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Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo
BACKGROUND: Goose parvovirus (GPV) is a Dependovirus associated with latent infection and mortality in geese. Currently, it severely affects geese production worldwide. The objective of this study was to develop a fluorescent quantitative real-time polymerase chain reaction (PCR) (FQ-PCR) assay for...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2751755/ https://www.ncbi.nlm.nih.gov/pubmed/19754946 http://dx.doi.org/10.1186/1743-422X-6-142 |
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author | Yang, Jin-Long Cheng, An-Chun Wang, Ming-Shu Pan, Kang-Cheng Li, Min Guo, Yu-Fei Li, Chuan-Feng Zhu, De-Kang Chen, Xiao-Yue |
author_facet | Yang, Jin-Long Cheng, An-Chun Wang, Ming-Shu Pan, Kang-Cheng Li, Min Guo, Yu-Fei Li, Chuan-Feng Zhu, De-Kang Chen, Xiao-Yue |
author_sort | Yang, Jin-Long |
collection | PubMed |
description | BACKGROUND: Goose parvovirus (GPV) is a Dependovirus associated with latent infection and mortality in geese. Currently, it severely affects geese production worldwide. The objective of this study was to develop a fluorescent quantitative real-time polymerase chain reaction (PCR) (FQ-PCR) assay for fast and accurate quantification of GPV DNA in infected goslings, which can aid in the understanding of the regular distribution pattern and the nosogenesis of GPV in vivo. RESULTS: The detection limit of the assay was 2.8 × 10(1 )standard DNA copies, with a sensitivity of 3 logs higher than that of the conventional gel-based PCR assay targeting the same gene. The real-time PCR was reproducible, as shown by satisfactory low intraassay and interassay coefficients of variation. CONCLUSION: The high sensitivity, specificity, simplicity, and reproducibility of the GPV fluorogenic PCR assay, combined with a high throughput, make this method suitable for a broad spectrum of GPV etiology-related applications. |
format | Text |
id | pubmed-2751755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27517552009-09-25 Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo Yang, Jin-Long Cheng, An-Chun Wang, Ming-Shu Pan, Kang-Cheng Li, Min Guo, Yu-Fei Li, Chuan-Feng Zhu, De-Kang Chen, Xiao-Yue Virol J Research BACKGROUND: Goose parvovirus (GPV) is a Dependovirus associated with latent infection and mortality in geese. Currently, it severely affects geese production worldwide. The objective of this study was to develop a fluorescent quantitative real-time polymerase chain reaction (PCR) (FQ-PCR) assay for fast and accurate quantification of GPV DNA in infected goslings, which can aid in the understanding of the regular distribution pattern and the nosogenesis of GPV in vivo. RESULTS: The detection limit of the assay was 2.8 × 10(1 )standard DNA copies, with a sensitivity of 3 logs higher than that of the conventional gel-based PCR assay targeting the same gene. The real-time PCR was reproducible, as shown by satisfactory low intraassay and interassay coefficients of variation. CONCLUSION: The high sensitivity, specificity, simplicity, and reproducibility of the GPV fluorogenic PCR assay, combined with a high throughput, make this method suitable for a broad spectrum of GPV etiology-related applications. BioMed Central 2009-09-15 /pmc/articles/PMC2751755/ /pubmed/19754946 http://dx.doi.org/10.1186/1743-422X-6-142 Text en Copyright © 2009 Yang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Yang, Jin-Long Cheng, An-Chun Wang, Ming-Shu Pan, Kang-Cheng Li, Min Guo, Yu-Fei Li, Chuan-Feng Zhu, De-Kang Chen, Xiao-Yue Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo |
title | Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo |
title_full | Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo |
title_fullStr | Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo |
title_full_unstemmed | Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo |
title_short | Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo |
title_sort | development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of goose parvovirus in vivo |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2751755/ https://www.ncbi.nlm.nih.gov/pubmed/19754946 http://dx.doi.org/10.1186/1743-422X-6-142 |
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