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Absolute Metabolite Concentrations and Implied Enzyme Active Site Occupancy in Escherichia coli
Absolute metabolite concentrations are critical to a quantitative understanding of cellular metabolism, as concentrations impact both the free energies and rates of metabolic reactions. Here we use liquid chromatography-tandem mass spectrometry to quantify more than 100 metabolite concentrations in...
| Autores principales: | , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2754216/ https://www.ncbi.nlm.nih.gov/pubmed/19561621 http://dx.doi.org/10.1038/nchembio.186 |