G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila

We combine Gal4/UAS, FLP/FRT and fluorescent reporters to generate cell clones that provide spatial, temporal, and genetic information about the origins of individual cells in Drosophila. We name this combination the Gal4 Technique for Real-time and Clonal Expression (G-TRACE). The approach should a...

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Autores principales: Evans, Cory J., Olson, John M., Ngo, Kathy T., Kim, Eunha, Lee, Noemi E., Kuoy, Edward, Patananan, Alexander N., Sitz, Daniel, Tran, PhuongThao, Do, Minh-Tu, Yackle, Kevin, Cespedes, Albert, Hartenstein, Volker, Call, Gerald B., Banerjee, Utpal
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2754220/
https://www.ncbi.nlm.nih.gov/pubmed/19633663
http://dx.doi.org/10.1038/nmeth.1356
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author Evans, Cory J.
Olson, John M.
Ngo, Kathy T.
Kim, Eunha
Lee, Noemi E.
Kuoy, Edward
Patananan, Alexander N.
Sitz, Daniel
Tran, PhuongThao
Do, Minh-Tu
Yackle, Kevin
Cespedes, Albert
Hartenstein, Volker
Call, Gerald B.
Banerjee, Utpal
author_facet Evans, Cory J.
Olson, John M.
Ngo, Kathy T.
Kim, Eunha
Lee, Noemi E.
Kuoy, Edward
Patananan, Alexander N.
Sitz, Daniel
Tran, PhuongThao
Do, Minh-Tu
Yackle, Kevin
Cespedes, Albert
Hartenstein, Volker
Call, Gerald B.
Banerjee, Utpal
author_sort Evans, Cory J.
collection PubMed
description We combine Gal4/UAS, FLP/FRT and fluorescent reporters to generate cell clones that provide spatial, temporal, and genetic information about the origins of individual cells in Drosophila. We name this combination the Gal4 Technique for Real-time and Clonal Expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.
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spelling pubmed-27542202010-02-01 G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila Evans, Cory J. Olson, John M. Ngo, Kathy T. Kim, Eunha Lee, Noemi E. Kuoy, Edward Patananan, Alexander N. Sitz, Daniel Tran, PhuongThao Do, Minh-Tu Yackle, Kevin Cespedes, Albert Hartenstein, Volker Call, Gerald B. Banerjee, Utpal Nat Methods Article We combine Gal4/UAS, FLP/FRT and fluorescent reporters to generate cell clones that provide spatial, temporal, and genetic information about the origins of individual cells in Drosophila. We name this combination the Gal4 Technique for Real-time and Clonal Expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale. 2009-07-26 2009-08 /pmc/articles/PMC2754220/ /pubmed/19633663 http://dx.doi.org/10.1038/nmeth.1356 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Evans, Cory J.
Olson, John M.
Ngo, Kathy T.
Kim, Eunha
Lee, Noemi E.
Kuoy, Edward
Patananan, Alexander N.
Sitz, Daniel
Tran, PhuongThao
Do, Minh-Tu
Yackle, Kevin
Cespedes, Albert
Hartenstein, Volker
Call, Gerald B.
Banerjee, Utpal
G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila
title G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila
title_full G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila
title_fullStr G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila
title_full_unstemmed G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila
title_short G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila
title_sort g-trace: rapid gal4-based cell lineage analysis in drosophila
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2754220/
https://www.ncbi.nlm.nih.gov/pubmed/19633663
http://dx.doi.org/10.1038/nmeth.1356
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