Cargando…

Presence of Helicobacter pylori in betel chewers and non betel chewers with and without oral cancers

BACKGROUND: Betel chewing has been shown to predispose to periodontal disease and oral cancer. Studies show that people with gum disease are more likely to test positive for Helicobacter pylori (H. pylori). It is not known if the lesions produced by betel quid and the resulting, chemical changes pre...

Descripción completa

Detalles Bibliográficos
Autores principales: Fernando, Neluka, Jayakumar, Gnanapragasam, Perera, Naomal, Amarasingha, Indranee, Meedin, Fahra, Holton, John
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2755467/
https://www.ncbi.nlm.nih.gov/pubmed/19772630
http://dx.doi.org/10.1186/1472-6831-9-23
_version_ 1782172449675149312
author Fernando, Neluka
Jayakumar, Gnanapragasam
Perera, Naomal
Amarasingha, Indranee
Meedin, Fahra
Holton, John
author_facet Fernando, Neluka
Jayakumar, Gnanapragasam
Perera, Naomal
Amarasingha, Indranee
Meedin, Fahra
Holton, John
author_sort Fernando, Neluka
collection PubMed
description BACKGROUND: Betel chewing has been shown to predispose to periodontal disease and oral cancer. Studies show that people with gum disease are more likely to test positive for Helicobacter pylori (H. pylori). It is not known if the lesions produced by betel quid and the resulting, chemical changes predispose to colonization by H. pylori. Further the role of this organism in oral cancer is not known. Our objective was to determine the presence of H. pylori in oral lesions of thirty oral cancer patients and to determine the presence of IgG antibodies to H. pylori in oral cancer patients who are betel chewers and non betel chewers, healthy betel chewers and healthy non-betel chewers and to compare the presence of H. pylori in these four groups. This case control study was conducted at the Cancer Institute Maharagama and the Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura. METHODS: One hundred and seventy three subjects, of whom fifty three were patients presenting with oral cancer to the Cancer Institute Maharagama, sixty healthy betel chewers and sixty healthy non-betel chewers from the Religious and Welfare Service Centre Maharagama were tested for H. pylori by serology. Thirty oral biopsies from oral cancer patients were cultured under microaerophilic condition to isolate H. pylori. The statistic used was Chi-square test. RESULTS: Of the fifty-three oral cancer patients, forty-four were betel chewers. Among the 53 oral cancer patients examined, ten of forty-four (10/44 = 22.7%) patients who are betel chewers and four of nine (4/9 = 44.4%) patients who are non-betel chewers were detected positive for IgG antibody against H. pylori. In the healthy group (betel chewers and non betel chewers) ten (16.7%) of the healthy betel chewers tested positive for H. pylori by serology. None of the healthy non-betel chewers tested positive for H. pylori Fourteen [26.4%] of oral cancer patients tested positive for H. pylori by serology, of which two were also culture positive (Only thirty samples were cultured). The presence of H. pylori in betel chewers (with or without cancer) compared to non-betel chewers was statistically significant. (Chi-square test p < 0.05) The use of tobacco and areca nut in betel chewers was significant with the presence of H. pylori (p < 0.05). CONCLUSION: There is a significant higher proportion of H. pylori in betel chewers compared to non-betel chewers but not between oral cancer patients compared to patients without oral cancer. Hence Betel chewing may predispose to colonisation with H. pylori in the digestive tract through swallowing the quid or during betel chewing.
format Text
id pubmed-2755467
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-27554672009-10-02 Presence of Helicobacter pylori in betel chewers and non betel chewers with and without oral cancers Fernando, Neluka Jayakumar, Gnanapragasam Perera, Naomal Amarasingha, Indranee Meedin, Fahra Holton, John BMC Oral Health Research Article BACKGROUND: Betel chewing has been shown to predispose to periodontal disease and oral cancer. Studies show that people with gum disease are more likely to test positive for Helicobacter pylori (H. pylori). It is not known if the lesions produced by betel quid and the resulting, chemical changes predispose to colonization by H. pylori. Further the role of this organism in oral cancer is not known. Our objective was to determine the presence of H. pylori in oral lesions of thirty oral cancer patients and to determine the presence of IgG antibodies to H. pylori in oral cancer patients who are betel chewers and non betel chewers, healthy betel chewers and healthy non-betel chewers and to compare the presence of H. pylori in these four groups. This case control study was conducted at the Cancer Institute Maharagama and the Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura. METHODS: One hundred and seventy three subjects, of whom fifty three were patients presenting with oral cancer to the Cancer Institute Maharagama, sixty healthy betel chewers and sixty healthy non-betel chewers from the Religious and Welfare Service Centre Maharagama were tested for H. pylori by serology. Thirty oral biopsies from oral cancer patients were cultured under microaerophilic condition to isolate H. pylori. The statistic used was Chi-square test. RESULTS: Of the fifty-three oral cancer patients, forty-four were betel chewers. Among the 53 oral cancer patients examined, ten of forty-four (10/44 = 22.7%) patients who are betel chewers and four of nine (4/9 = 44.4%) patients who are non-betel chewers were detected positive for IgG antibody against H. pylori. In the healthy group (betel chewers and non betel chewers) ten (16.7%) of the healthy betel chewers tested positive for H. pylori by serology. None of the healthy non-betel chewers tested positive for H. pylori Fourteen [26.4%] of oral cancer patients tested positive for H. pylori by serology, of which two were also culture positive (Only thirty samples were cultured). The presence of H. pylori in betel chewers (with or without cancer) compared to non-betel chewers was statistically significant. (Chi-square test p < 0.05) The use of tobacco and areca nut in betel chewers was significant with the presence of H. pylori (p < 0.05). CONCLUSION: There is a significant higher proportion of H. pylori in betel chewers compared to non-betel chewers but not between oral cancer patients compared to patients without oral cancer. Hence Betel chewing may predispose to colonisation with H. pylori in the digestive tract through swallowing the quid or during betel chewing. BioMed Central 2009-09-22 /pmc/articles/PMC2755467/ /pubmed/19772630 http://dx.doi.org/10.1186/1472-6831-9-23 Text en Copyright © 2009 Fernando et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Fernando, Neluka
Jayakumar, Gnanapragasam
Perera, Naomal
Amarasingha, Indranee
Meedin, Fahra
Holton, John
Presence of Helicobacter pylori in betel chewers and non betel chewers with and without oral cancers
title Presence of Helicobacter pylori in betel chewers and non betel chewers with and without oral cancers
title_full Presence of Helicobacter pylori in betel chewers and non betel chewers with and without oral cancers
title_fullStr Presence of Helicobacter pylori in betel chewers and non betel chewers with and without oral cancers
title_full_unstemmed Presence of Helicobacter pylori in betel chewers and non betel chewers with and without oral cancers
title_short Presence of Helicobacter pylori in betel chewers and non betel chewers with and without oral cancers
title_sort presence of helicobacter pylori in betel chewers and non betel chewers with and without oral cancers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2755467/
https://www.ncbi.nlm.nih.gov/pubmed/19772630
http://dx.doi.org/10.1186/1472-6831-9-23
work_keys_str_mv AT fernandoneluka presenceofhelicobacterpyloriinbetelchewersandnonbetelchewerswithandwithoutoralcancers
AT jayakumargnanapragasam presenceofhelicobacterpyloriinbetelchewersandnonbetelchewerswithandwithoutoralcancers
AT pereranaomal presenceofhelicobacterpyloriinbetelchewersandnonbetelchewerswithandwithoutoralcancers
AT amarasinghaindranee presenceofhelicobacterpyloriinbetelchewersandnonbetelchewerswithandwithoutoralcancers
AT meedinfahra presenceofhelicobacterpyloriinbetelchewersandnonbetelchewerswithandwithoutoralcancers
AT holtonjohn presenceofhelicobacterpyloriinbetelchewersandnonbetelchewerswithandwithoutoralcancers