Nanoscale 3D cellular imaging by axial scanning transmission electron tomography

Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We show that scanning transmission e...

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Detalles Bibliográficos
Autores principales: Hohmann-Marriott, Martin F., Sousa, Alioscka A., Azari, Afrouz A., Glushakova, Svetlana, Zhang, Guofeng, Zimmerberg, Joshua, Leapman, Richard D.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2755602/
https://www.ncbi.nlm.nih.gov/pubmed/19718033
http://dx.doi.org/10.1038/nmeth.1367
Descripción
Sumario:Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We show that scanning transmission electron tomography of 1000 nm thick samples using axial detection provides resolution comparable to conventional electron tomography. The method is demonstrated by reconstructing a human erythrocyte infected with the malaria parasite Plasmodium falciparum.

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