Nanoscale 3D cellular imaging by axial scanning transmission electron tomography

Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We show that scanning transmission e...

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Detalles Bibliográficos
Autores principales: Hohmann-Marriott, Martin F., Sousa, Alioscka A., Azari, Afrouz A., Glushakova, Svetlana, Zhang, Guofeng, Zimmerberg, Joshua, Leapman, Richard D.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2755602/
https://www.ncbi.nlm.nih.gov/pubmed/19718033
http://dx.doi.org/10.1038/nmeth.1367
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author Hohmann-Marriott, Martin F.
Sousa, Alioscka A.
Azari, Afrouz A.
Glushakova, Svetlana
Zhang, Guofeng
Zimmerberg, Joshua
Leapman, Richard D.
author_facet Hohmann-Marriott, Martin F.
Sousa, Alioscka A.
Azari, Afrouz A.
Glushakova, Svetlana
Zhang, Guofeng
Zimmerberg, Joshua
Leapman, Richard D.
author_sort Hohmann-Marriott, Martin F.
collection PubMed
description Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We show that scanning transmission electron tomography of 1000 nm thick samples using axial detection provides resolution comparable to conventional electron tomography. The method is demonstrated by reconstructing a human erythrocyte infected with the malaria parasite Plasmodium falciparum.
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spelling pubmed-27556022010-04-01 Nanoscale 3D cellular imaging by axial scanning transmission electron tomography Hohmann-Marriott, Martin F. Sousa, Alioscka A. Azari, Afrouz A. Glushakova, Svetlana Zhang, Guofeng Zimmerberg, Joshua Leapman, Richard D. Nat Methods Article Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We show that scanning transmission electron tomography of 1000 nm thick samples using axial detection provides resolution comparable to conventional electron tomography. The method is demonstrated by reconstructing a human erythrocyte infected with the malaria parasite Plasmodium falciparum. 2009-08-30 2009-10 /pmc/articles/PMC2755602/ /pubmed/19718033 http://dx.doi.org/10.1038/nmeth.1367 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Hohmann-Marriott, Martin F.
Sousa, Alioscka A.
Azari, Afrouz A.
Glushakova, Svetlana
Zhang, Guofeng
Zimmerberg, Joshua
Leapman, Richard D.
Nanoscale 3D cellular imaging by axial scanning transmission electron tomography
title Nanoscale 3D cellular imaging by axial scanning transmission electron tomography
title_full Nanoscale 3D cellular imaging by axial scanning transmission electron tomography
title_fullStr Nanoscale 3D cellular imaging by axial scanning transmission electron tomography
title_full_unstemmed Nanoscale 3D cellular imaging by axial scanning transmission electron tomography
title_short Nanoscale 3D cellular imaging by axial scanning transmission electron tomography
title_sort nanoscale 3d cellular imaging by axial scanning transmission electron tomography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2755602/
https://www.ncbi.nlm.nih.gov/pubmed/19718033
http://dx.doi.org/10.1038/nmeth.1367
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