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Morphological, physiological and behavioural evaluation of a ‘Mice in Space’ housing system

Environmental conditions likely affect physiology and behaviour of mice used for life sciences research on Earth or in Space. Here, we analysed the effects of cage confinement on the weightbearing musculoskeletal system, behaviour and stress of wild-type mice (C57BL/6JRj, 30 g b.wt., total n = 24) h...

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Autores principales: Blottner, Dieter, Serradj, Najet, Salanova, Michele, Touma, Chadi, Palme, Rupert, Silva, Mitchell, Aerts, Jean Marie, Berckmans, Daniel, Vico, Laurence, Liu, Yi, Giuliani, Alessandra, Rustichelli, Franco, Cancedda, Ranieri, Jamon, Marc
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2755731/
https://www.ncbi.nlm.nih.gov/pubmed/19130060
http://dx.doi.org/10.1007/s00360-008-0330-4
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author Blottner, Dieter
Serradj, Najet
Salanova, Michele
Touma, Chadi
Palme, Rupert
Silva, Mitchell
Aerts, Jean Marie
Berckmans, Daniel
Vico, Laurence
Liu, Yi
Giuliani, Alessandra
Rustichelli, Franco
Cancedda, Ranieri
Jamon, Marc
author_facet Blottner, Dieter
Serradj, Najet
Salanova, Michele
Touma, Chadi
Palme, Rupert
Silva, Mitchell
Aerts, Jean Marie
Berckmans, Daniel
Vico, Laurence
Liu, Yi
Giuliani, Alessandra
Rustichelli, Franco
Cancedda, Ranieri
Jamon, Marc
author_sort Blottner, Dieter
collection PubMed
description Environmental conditions likely affect physiology and behaviour of mice used for life sciences research on Earth or in Space. Here, we analysed the effects of cage confinement on the weightbearing musculoskeletal system, behaviour and stress of wild-type mice (C57BL/6JRj, 30 g b.wt., total n = 24) housed for 25 days in a prototypical ground-based and fully automated life support habitat device called “Mice in Space” (MIS). Compared with control housing (individually ventilated cages) the MIS mice revealed no significant changes in soleus muscle size and myofiber distribution (type I vs. II) and quality of bone (3-D microarchitecture and mineralisation of calvaria, spine and femur) determined by confocal and micro-computed tomography. Corticosterone metabolism measured non-invasively (faeces) monitored elevated adrenocortical activity at only start of the MIS cage confinement (day 1). Behavioural tests (i.e., grip strength, rotarod, L/D box, elevated plus-maze, open field, aggressiveness) performed subsequently revealed only minor changes in motor performance (MIS vs. controls). The MIS habitat will not, on its own, produce major effects that could confound interpretation of data induced by microgravity exposure during spaceflight. Our results may be even more helpful in developing multidisciplinary protocols with adequate scenarios addressing molecular to systems levels using mice of various genetic phenotypes in many laboratories. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00360-008-0330-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-27557312009-10-07 Morphological, physiological and behavioural evaluation of a ‘Mice in Space’ housing system Blottner, Dieter Serradj, Najet Salanova, Michele Touma, Chadi Palme, Rupert Silva, Mitchell Aerts, Jean Marie Berckmans, Daniel Vico, Laurence Liu, Yi Giuliani, Alessandra Rustichelli, Franco Cancedda, Ranieri Jamon, Marc J Comp Physiol B Original Paper Environmental conditions likely affect physiology and behaviour of mice used for life sciences research on Earth or in Space. Here, we analysed the effects of cage confinement on the weightbearing musculoskeletal system, behaviour and stress of wild-type mice (C57BL/6JRj, 30 g b.wt., total n = 24) housed for 25 days in a prototypical ground-based and fully automated life support habitat device called “Mice in Space” (MIS). Compared with control housing (individually ventilated cages) the MIS mice revealed no significant changes in soleus muscle size and myofiber distribution (type I vs. II) and quality of bone (3-D microarchitecture and mineralisation of calvaria, spine and femur) determined by confocal and micro-computed tomography. Corticosterone metabolism measured non-invasively (faeces) monitored elevated adrenocortical activity at only start of the MIS cage confinement (day 1). Behavioural tests (i.e., grip strength, rotarod, L/D box, elevated plus-maze, open field, aggressiveness) performed subsequently revealed only minor changes in motor performance (MIS vs. controls). The MIS habitat will not, on its own, produce major effects that could confound interpretation of data induced by microgravity exposure during spaceflight. Our results may be even more helpful in developing multidisciplinary protocols with adequate scenarios addressing molecular to systems levels using mice of various genetic phenotypes in many laboratories. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00360-008-0330-4) contains supplementary material, which is available to authorized users. Springer-Verlag 2009-01-08 2009-05 /pmc/articles/PMC2755731/ /pubmed/19130060 http://dx.doi.org/10.1007/s00360-008-0330-4 Text en © Springer-Verlag 2009
spellingShingle Original Paper
Blottner, Dieter
Serradj, Najet
Salanova, Michele
Touma, Chadi
Palme, Rupert
Silva, Mitchell
Aerts, Jean Marie
Berckmans, Daniel
Vico, Laurence
Liu, Yi
Giuliani, Alessandra
Rustichelli, Franco
Cancedda, Ranieri
Jamon, Marc
Morphological, physiological and behavioural evaluation of a ‘Mice in Space’ housing system
title Morphological, physiological and behavioural evaluation of a ‘Mice in Space’ housing system
title_full Morphological, physiological and behavioural evaluation of a ‘Mice in Space’ housing system
title_fullStr Morphological, physiological and behavioural evaluation of a ‘Mice in Space’ housing system
title_full_unstemmed Morphological, physiological and behavioural evaluation of a ‘Mice in Space’ housing system
title_short Morphological, physiological and behavioural evaluation of a ‘Mice in Space’ housing system
title_sort morphological, physiological and behavioural evaluation of a ‘mice in space’ housing system
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2755731/
https://www.ncbi.nlm.nih.gov/pubmed/19130060
http://dx.doi.org/10.1007/s00360-008-0330-4
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