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Expression patterns of the aquaporin gene family during renal development: influence of genetic variability

High-throughput analyses have shown that aquaporins (AQPs) belong to a cluster of genes that are differentially expressed during kidney organogenesis. However, the spatiotemporal expression patterns of the AQP gene family during tubular maturation and the potential influence of genetic variation on...

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Autores principales: Parreira, Kleber S., Debaix, Huguette, Cnops, Yvette, Geffers, Lars, Devuyst, Olivier
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756349/
https://www.ncbi.nlm.nih.gov/pubmed/19367412
http://dx.doi.org/10.1007/s00424-009-0667-x
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author Parreira, Kleber S.
Debaix, Huguette
Cnops, Yvette
Geffers, Lars
Devuyst, Olivier
author_facet Parreira, Kleber S.
Debaix, Huguette
Cnops, Yvette
Geffers, Lars
Devuyst, Olivier
author_sort Parreira, Kleber S.
collection PubMed
description High-throughput analyses have shown that aquaporins (AQPs) belong to a cluster of genes that are differentially expressed during kidney organogenesis. However, the spatiotemporal expression patterns of the AQP gene family during tubular maturation and the potential influence of genetic variation on these patterns and on water handling remain unknown. We investigated the expression patterns of all AQP isoforms in fetal (E13.5 to E18.5), postnatal (P1 to P28), and adult (9 weeks) kidneys of inbred (C57BL/6J) and outbred (CD-1) mice. Using quantitative polymerase chain reaction (PCR), we evidenced two mRNA patterns during tubular maturation in C57 mice. The AQPs 1-7-11 showed an early (from E14.5) and progressive increase to adult levels, similar to the mRNA pattern observed for proximal tubule markers (Megalin, NaPi-IIa, OAT1) and reflecting the continuous increase in renal cortical structures during development. By contrast, AQPs 2-3-4 showed a later (E15.5) and more abrupt increase, with transient postnatal overexpression. Most AQP genes were expressed earlier and/or stronger in maturing CD-1 kidneys. Furthermore, adult CD-1 kidneys expressed more AQP2 in the collecting ducts, which was reflected by a significant delay in excreting a water load. The expression patterns of proximal vs. distal AQPs and the earlier expression in the CD-1 strain were confirmed by immunoblotting and immunostaining. These data (1) substantiate the clustering of important genes during tubular maturation and (2) demonstrate that genetic variability influences the regulation of the AQP gene family during tubular maturation and water handling by the mature kidney. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00424-009-0667-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-27563492009-10-07 Expression patterns of the aquaporin gene family during renal development: influence of genetic variability Parreira, Kleber S. Debaix, Huguette Cnops, Yvette Geffers, Lars Devuyst, Olivier Pflugers Arch Molecular and Genomic Physiology High-throughput analyses have shown that aquaporins (AQPs) belong to a cluster of genes that are differentially expressed during kidney organogenesis. However, the spatiotemporal expression patterns of the AQP gene family during tubular maturation and the potential influence of genetic variation on these patterns and on water handling remain unknown. We investigated the expression patterns of all AQP isoforms in fetal (E13.5 to E18.5), postnatal (P1 to P28), and adult (9 weeks) kidneys of inbred (C57BL/6J) and outbred (CD-1) mice. Using quantitative polymerase chain reaction (PCR), we evidenced two mRNA patterns during tubular maturation in C57 mice. The AQPs 1-7-11 showed an early (from E14.5) and progressive increase to adult levels, similar to the mRNA pattern observed for proximal tubule markers (Megalin, NaPi-IIa, OAT1) and reflecting the continuous increase in renal cortical structures during development. By contrast, AQPs 2-3-4 showed a later (E15.5) and more abrupt increase, with transient postnatal overexpression. Most AQP genes were expressed earlier and/or stronger in maturing CD-1 kidneys. Furthermore, adult CD-1 kidneys expressed more AQP2 in the collecting ducts, which was reflected by a significant delay in excreting a water load. The expression patterns of proximal vs. distal AQPs and the earlier expression in the CD-1 strain were confirmed by immunoblotting and immunostaining. These data (1) substantiate the clustering of important genes during tubular maturation and (2) demonstrate that genetic variability influences the regulation of the AQP gene family during tubular maturation and water handling by the mature kidney. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00424-009-0667-x) contains supplementary material, which is available to authorized users. Springer-Verlag 2009-04-16 2009-08 /pmc/articles/PMC2756349/ /pubmed/19367412 http://dx.doi.org/10.1007/s00424-009-0667-x Text en © Springer-Verlag 2009
spellingShingle Molecular and Genomic Physiology
Parreira, Kleber S.
Debaix, Huguette
Cnops, Yvette
Geffers, Lars
Devuyst, Olivier
Expression patterns of the aquaporin gene family during renal development: influence of genetic variability
title Expression patterns of the aquaporin gene family during renal development: influence of genetic variability
title_full Expression patterns of the aquaporin gene family during renal development: influence of genetic variability
title_fullStr Expression patterns of the aquaporin gene family during renal development: influence of genetic variability
title_full_unstemmed Expression patterns of the aquaporin gene family during renal development: influence of genetic variability
title_short Expression patterns of the aquaporin gene family during renal development: influence of genetic variability
title_sort expression patterns of the aquaporin gene family during renal development: influence of genetic variability
topic Molecular and Genomic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756349/
https://www.ncbi.nlm.nih.gov/pubmed/19367412
http://dx.doi.org/10.1007/s00424-009-0667-x
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