Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells

Recent studies have indicated that nuclear protein of 95 kDa (Np95) is essential for maintaining genomic methylation by recruiting DNA methyltransferase (Dnmt) 1 to hemi-methylated sites. Here, we show that Np95 interacts more strongly with regulatory domains of the de novo methyltransferases Dnmt3a...

Descripción completa

Detalles Bibliográficos
Autores principales: Meilinger, Daniela, Fellinger, Karin, Bultmann, Sebastian, Rothbauer, Ulrich, Bonapace, Ian Marc, Klinkert, Wolfgang E F, Spada, Fabio, Leonhardt, Heinrich
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2009
Materias:
Scientific Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756565/
https://www.ncbi.nlm.nih.gov/pubmed/19798101
http://dx.doi.org/10.1038/embor.2009.201
Descripción
Sumario:Recent studies have indicated that nuclear protein of 95 kDa (Np95) is essential for maintaining genomic methylation by recruiting DNA methyltransferase (Dnmt) 1 to hemi-methylated sites. Here, we show that Np95 interacts more strongly with regulatory domains of the de novo methyltransferases Dnmt3a and Dnmt3b. To investigate possible functions, we developed an epigenetic silencing assay using fluorescent reporters in embryonic stem cells (ESCs). Interestingly, silencing of the cytomegalovirus promoter in ESCs preceded DNA methylation and was strictly dependent on the presence of either Np95, histone H3 methyltransferase G9a or Dnmt3a and Dnmt3b. Our results indicate a regulatory role for Np95, Dnmt3a and Dnmt3b in mediating epigenetic silencing through histone modification followed by DNA methylation.

Ejemplares similares