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Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells
Recent studies have indicated that nuclear protein of 95 kDa (Np95) is essential for maintaining genomic methylation by recruiting DNA methyltransferase (Dnmt) 1 to hemi-methylated sites. Here, we show that Np95 interacts more strongly with regulatory domains of the de novo methyltransferases Dnmt3a...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756565/ https://www.ncbi.nlm.nih.gov/pubmed/19798101 http://dx.doi.org/10.1038/embor.2009.201 |
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author | Meilinger, Daniela Fellinger, Karin Bultmann, Sebastian Rothbauer, Ulrich Bonapace, Ian Marc Klinkert, Wolfgang E F Spada, Fabio Leonhardt, Heinrich |
author_facet | Meilinger, Daniela Fellinger, Karin Bultmann, Sebastian Rothbauer, Ulrich Bonapace, Ian Marc Klinkert, Wolfgang E F Spada, Fabio Leonhardt, Heinrich |
author_sort | Meilinger, Daniela |
collection | PubMed |
description | Recent studies have indicated that nuclear protein of 95 kDa (Np95) is essential for maintaining genomic methylation by recruiting DNA methyltransferase (Dnmt) 1 to hemi-methylated sites. Here, we show that Np95 interacts more strongly with regulatory domains of the de novo methyltransferases Dnmt3a and Dnmt3b. To investigate possible functions, we developed an epigenetic silencing assay using fluorescent reporters in embryonic stem cells (ESCs). Interestingly, silencing of the cytomegalovirus promoter in ESCs preceded DNA methylation and was strictly dependent on the presence of either Np95, histone H3 methyltransferase G9a or Dnmt3a and Dnmt3b. Our results indicate a regulatory role for Np95, Dnmt3a and Dnmt3b in mediating epigenetic silencing through histone modification followed by DNA methylation. |
format | Text |
id | pubmed-2756565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-27565652009-10-08 Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells Meilinger, Daniela Fellinger, Karin Bultmann, Sebastian Rothbauer, Ulrich Bonapace, Ian Marc Klinkert, Wolfgang E F Spada, Fabio Leonhardt, Heinrich EMBO Rep Scientific Report Recent studies have indicated that nuclear protein of 95 kDa (Np95) is essential for maintaining genomic methylation by recruiting DNA methyltransferase (Dnmt) 1 to hemi-methylated sites. Here, we show that Np95 interacts more strongly with regulatory domains of the de novo methyltransferases Dnmt3a and Dnmt3b. To investigate possible functions, we developed an epigenetic silencing assay using fluorescent reporters in embryonic stem cells (ESCs). Interestingly, silencing of the cytomegalovirus promoter in ESCs preceded DNA methylation and was strictly dependent on the presence of either Np95, histone H3 methyltransferase G9a or Dnmt3a and Dnmt3b. Our results indicate a regulatory role for Np95, Dnmt3a and Dnmt3b in mediating epigenetic silencing through histone modification followed by DNA methylation. Nature Publishing Group 2009-11 2009-10-02 /pmc/articles/PMC2756565/ /pubmed/19798101 http://dx.doi.org/10.1038/embor.2009.201 Text en Copyright © 2009, European Molecular Biology Organization http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation without specific permission. |
spellingShingle | Scientific Report Meilinger, Daniela Fellinger, Karin Bultmann, Sebastian Rothbauer, Ulrich Bonapace, Ian Marc Klinkert, Wolfgang E F Spada, Fabio Leonhardt, Heinrich Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells |
title | Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells |
title_full | Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells |
title_fullStr | Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells |
title_full_unstemmed | Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells |
title_short | Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells |
title_sort | np95 interacts with de novo dna methyltransferases, dnmt3a and dnmt3b, and mediates epigenetic silencing of the viral cmv promoter in embryonic stem cells |
topic | Scientific Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756565/ https://www.ncbi.nlm.nih.gov/pubmed/19798101 http://dx.doi.org/10.1038/embor.2009.201 |
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