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A switchable light-input, light-output system modelled and constructed in yeast

BACKGROUND: Advances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidopsis proteins PHYTOCHROME A and FAR-RED ELONGATED HYPOCOTYL 1-LIKE. Light input...

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Autores principales: Sorokina, Oxana, Kapus, Anita, Terecskei, Kata, Dixon, Laura E, Kozma-Bognar, Laszlo, Nagy, Ferenc, Millar, Andrew J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2758823/
https://www.ncbi.nlm.nih.gov/pubmed/19761615
http://dx.doi.org/10.1186/1754-1611-3-15
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author Sorokina, Oxana
Kapus, Anita
Terecskei, Kata
Dixon, Laura E
Kozma-Bognar, Laszlo
Nagy, Ferenc
Millar, Andrew J
author_facet Sorokina, Oxana
Kapus, Anita
Terecskei, Kata
Dixon, Laura E
Kozma-Bognar, Laszlo
Nagy, Ferenc
Millar, Andrew J
author_sort Sorokina, Oxana
collection PubMed
description BACKGROUND: Advances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidopsis proteins PHYTOCHROME A and FAR-RED ELONGATED HYPOCOTYL 1-LIKE. Light input to this regulatory module allows dynamic control of a light-emitting LUCIFERASE reporter gene, which we detect by real-time imaging of yeast colonies on solid media. RESULTS: The reversible activation of the phytochrome by red light, and its inactivation by far-red light, is retained. We use this quantitative readout to construct a mathematical model that matches the system's behaviour and predicts the molecular targets for future manipulation. CONCLUSION: Our model, methods and materials together constitute a novel system for a eukaryotic host with the potential to convert a dynamic pattern of light input into a predictable gene expression response. This system could be applied for the regulation of genetic networks - both known and synthetic.
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spelling pubmed-27588232009-10-08 A switchable light-input, light-output system modelled and constructed in yeast Sorokina, Oxana Kapus, Anita Terecskei, Kata Dixon, Laura E Kozma-Bognar, Laszlo Nagy, Ferenc Millar, Andrew J J Biol Eng Research BACKGROUND: Advances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidopsis proteins PHYTOCHROME A and FAR-RED ELONGATED HYPOCOTYL 1-LIKE. Light input to this regulatory module allows dynamic control of a light-emitting LUCIFERASE reporter gene, which we detect by real-time imaging of yeast colonies on solid media. RESULTS: The reversible activation of the phytochrome by red light, and its inactivation by far-red light, is retained. We use this quantitative readout to construct a mathematical model that matches the system's behaviour and predicts the molecular targets for future manipulation. CONCLUSION: Our model, methods and materials together constitute a novel system for a eukaryotic host with the potential to convert a dynamic pattern of light input into a predictable gene expression response. This system could be applied for the regulation of genetic networks - both known and synthetic. BioMed Central 2009-09-17 /pmc/articles/PMC2758823/ /pubmed/19761615 http://dx.doi.org/10.1186/1754-1611-3-15 Text en Copyright © 2009 Sorokina et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Sorokina, Oxana
Kapus, Anita
Terecskei, Kata
Dixon, Laura E
Kozma-Bognar, Laszlo
Nagy, Ferenc
Millar, Andrew J
A switchable light-input, light-output system modelled and constructed in yeast
title A switchable light-input, light-output system modelled and constructed in yeast
title_full A switchable light-input, light-output system modelled and constructed in yeast
title_fullStr A switchable light-input, light-output system modelled and constructed in yeast
title_full_unstemmed A switchable light-input, light-output system modelled and constructed in yeast
title_short A switchable light-input, light-output system modelled and constructed in yeast
title_sort switchable light-input, light-output system modelled and constructed in yeast
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2758823/
https://www.ncbi.nlm.nih.gov/pubmed/19761615
http://dx.doi.org/10.1186/1754-1611-3-15
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