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Abnormal IgD and IgA1 O-glycosylation in hyperimmunoglobulinaemia D and periodic fever syndrome

In order to determine the glycosylation pattern for IgD, and to examine whether there are changes in the pattern of IgD and IgA1 O-glycosylation in patients with hyperimmunoglobulinaemia D and periodic fever syndrome (HIDS) during acute febrile attacks and during periods of quiescence, serum was obt...

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Autores principales: de Wolff, Jacob F., Dickinson, Stephen J., Smith, Alice C., Molyneux, Karen, Feehally, John, Simon, Anna, Barratt, Jonathan
Formato: Texto
Lenguaje:English
Publicado: Springer Milan 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2759005/
https://www.ncbi.nlm.nih.gov/pubmed/19543954
http://dx.doi.org/10.1007/s10238-009-0056-y
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author de Wolff, Jacob F.
Dickinson, Stephen J.
Smith, Alice C.
Molyneux, Karen
Feehally, John
Simon, Anna
Barratt, Jonathan
author_facet de Wolff, Jacob F.
Dickinson, Stephen J.
Smith, Alice C.
Molyneux, Karen
Feehally, John
Simon, Anna
Barratt, Jonathan
author_sort de Wolff, Jacob F.
collection PubMed
description In order to determine the glycosylation pattern for IgD, and to examine whether there are changes in the pattern of IgD and IgA1 O-glycosylation in patients with hyperimmunoglobulinaemia D and periodic fever syndrome (HIDS) during acute febrile attacks and during periods of quiescence, serum was obtained from 20 patients with HIDS and 20 control subjects. In the HIDS group, serum was obtained either during an acute febrile episode (n = 9) or during a period of quiescence (n = 11). The O-glycosylation profiles of native and desialylated IgA1 and IgD were measured in an ELISA-type system using the lectins Helix aspersa and peanut agglutinin, which bind to alternative forms of O-glycan moieties. IgD is more heavily O-galactosylated and less O-sialylated than IgA1 in healthy subjects. HIDS is associated with more extensive O-galactosylation of IgD and a reduction in O-sialylation of both IgD and IgA1. These changes are present both during acute febrile attacks and periods of quiescence. The T cell IgD receptor is a lectin with binding affinity for the O-glycans of both IgD and IgA1. The observed changes in IgD and IgA1 O-glycosylation are likely to have a significant effect on IgD/IgA1–T cell IgD receptor interactions including basal immunoglobulin synthesis, and possibly myeloid IgD receptor-mediated cytokine release.
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spelling pubmed-27590052009-10-09 Abnormal IgD and IgA1 O-glycosylation in hyperimmunoglobulinaemia D and periodic fever syndrome de Wolff, Jacob F. Dickinson, Stephen J. Smith, Alice C. Molyneux, Karen Feehally, John Simon, Anna Barratt, Jonathan Clin Exp Med Original Article In order to determine the glycosylation pattern for IgD, and to examine whether there are changes in the pattern of IgD and IgA1 O-glycosylation in patients with hyperimmunoglobulinaemia D and periodic fever syndrome (HIDS) during acute febrile attacks and during periods of quiescence, serum was obtained from 20 patients with HIDS and 20 control subjects. In the HIDS group, serum was obtained either during an acute febrile episode (n = 9) or during a period of quiescence (n = 11). The O-glycosylation profiles of native and desialylated IgA1 and IgD were measured in an ELISA-type system using the lectins Helix aspersa and peanut agglutinin, which bind to alternative forms of O-glycan moieties. IgD is more heavily O-galactosylated and less O-sialylated than IgA1 in healthy subjects. HIDS is associated with more extensive O-galactosylation of IgD and a reduction in O-sialylation of both IgD and IgA1. These changes are present both during acute febrile attacks and periods of quiescence. The T cell IgD receptor is a lectin with binding affinity for the O-glycans of both IgD and IgA1. The observed changes in IgD and IgA1 O-glycosylation are likely to have a significant effect on IgD/IgA1–T cell IgD receptor interactions including basal immunoglobulin synthesis, and possibly myeloid IgD receptor-mediated cytokine release. Springer Milan 2009-06-20 2009-12 /pmc/articles/PMC2759005/ /pubmed/19543954 http://dx.doi.org/10.1007/s10238-009-0056-y Text en © The Author(s) 2009
spellingShingle Original Article
de Wolff, Jacob F.
Dickinson, Stephen J.
Smith, Alice C.
Molyneux, Karen
Feehally, John
Simon, Anna
Barratt, Jonathan
Abnormal IgD and IgA1 O-glycosylation in hyperimmunoglobulinaemia D and periodic fever syndrome
title Abnormal IgD and IgA1 O-glycosylation in hyperimmunoglobulinaemia D and periodic fever syndrome
title_full Abnormal IgD and IgA1 O-glycosylation in hyperimmunoglobulinaemia D and periodic fever syndrome
title_fullStr Abnormal IgD and IgA1 O-glycosylation in hyperimmunoglobulinaemia D and periodic fever syndrome
title_full_unstemmed Abnormal IgD and IgA1 O-glycosylation in hyperimmunoglobulinaemia D and periodic fever syndrome
title_short Abnormal IgD and IgA1 O-glycosylation in hyperimmunoglobulinaemia D and periodic fever syndrome
title_sort abnormal igd and iga1 o-glycosylation in hyperimmunoglobulinaemia d and periodic fever syndrome
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2759005/
https://www.ncbi.nlm.nih.gov/pubmed/19543954
http://dx.doi.org/10.1007/s10238-009-0056-y
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