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Combination of Nanoindentation and Quantitative Backscattered Electron Imaging Revealed Altered Bone Material Properties Associated with Femoral Neck Fragility

Osteoporotic fragility fractures were hypothesized to be related to changes in bone material properties and not solely to reduction in bone mass. We studied cortical bone from the superior and inferior sectors of whole femoral neck sections from five female osteoporotic hip fracture cases (74–92 yea...

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Autores principales: Fratzl-Zelman, N., Roschger, P., Gourrier, A., Weber, M., Misof, B. M., Loveridge, N., Reeve, J., Klaushofer, K., Fratzl, P.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2759010/
https://www.ncbi.nlm.nih.gov/pubmed/19756347
http://dx.doi.org/10.1007/s00223-009-9289-8
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author Fratzl-Zelman, N.
Roschger, P.
Gourrier, A.
Weber, M.
Misof, B. M.
Loveridge, N.
Reeve, J.
Klaushofer, K.
Fratzl, P.
author_facet Fratzl-Zelman, N.
Roschger, P.
Gourrier, A.
Weber, M.
Misof, B. M.
Loveridge, N.
Reeve, J.
Klaushofer, K.
Fratzl, P.
author_sort Fratzl-Zelman, N.
collection PubMed
description Osteoporotic fragility fractures were hypothesized to be related to changes in bone material properties and not solely to reduction in bone mass. We studied cortical bone from the superior and inferior sectors of whole femoral neck sections from five female osteoporotic hip fracture cases (74–92 years) and five nonfractured controls (75–88 years). The typical calcium content (Ca(Peak)) and the mineral particle thickness parameter (T) were mapped in large areas of the superior and inferior regions using quantitative backscattered electron imaging (qBEI) and scanning small-angle X-ray scattering, respectively. Additionally, indentation modulus (E) and hardness (H) (determined by nanoindentation) were compared at the local level to the mineral content (Ca(Ind)) at the indent positions (obtained from qBEI). Ca(Peak) (−2.2%, P = 0.002), Ca(Ind) (−1.8%, P = 0.048), E (−5.6%, P = 0.040), and H (−6.0%, P = 0.016) were significantly lower for the superior compared to the inferior region. Interestingly, Ca(Peak) as well as Ca(Ind) were also lower (−2.6%, P = 0.006, and –3.7%, P = 0.002, respectively) in fracture cases compared to controls, while E and H did not show any significant reduction. T values were in the normal range, independent of region (P = 0.181) or fracture status (P = 0.551). In conclusion, it appears that the observed femoral neck fragility is associated with a reduced mineral content, which was not accompanied by a reduction in stiffness and hardness of the bone material. This pilot study suggests that a stiffening process in the organic matrix component contributes to bone fragility independently of mineral content.
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spelling pubmed-27590102009-10-09 Combination of Nanoindentation and Quantitative Backscattered Electron Imaging Revealed Altered Bone Material Properties Associated with Femoral Neck Fragility Fratzl-Zelman, N. Roschger, P. Gourrier, A. Weber, M. Misof, B. M. Loveridge, N. Reeve, J. Klaushofer, K. Fratzl, P. Calcif Tissue Int Article Osteoporotic fragility fractures were hypothesized to be related to changes in bone material properties and not solely to reduction in bone mass. We studied cortical bone from the superior and inferior sectors of whole femoral neck sections from five female osteoporotic hip fracture cases (74–92 years) and five nonfractured controls (75–88 years). The typical calcium content (Ca(Peak)) and the mineral particle thickness parameter (T) were mapped in large areas of the superior and inferior regions using quantitative backscattered electron imaging (qBEI) and scanning small-angle X-ray scattering, respectively. Additionally, indentation modulus (E) and hardness (H) (determined by nanoindentation) were compared at the local level to the mineral content (Ca(Ind)) at the indent positions (obtained from qBEI). Ca(Peak) (−2.2%, P = 0.002), Ca(Ind) (−1.8%, P = 0.048), E (−5.6%, P = 0.040), and H (−6.0%, P = 0.016) were significantly lower for the superior compared to the inferior region. Interestingly, Ca(Peak) as well as Ca(Ind) were also lower (−2.6%, P = 0.006, and –3.7%, P = 0.002, respectively) in fracture cases compared to controls, while E and H did not show any significant reduction. T values were in the normal range, independent of region (P = 0.181) or fracture status (P = 0.551). In conclusion, it appears that the observed femoral neck fragility is associated with a reduced mineral content, which was not accompanied by a reduction in stiffness and hardness of the bone material. This pilot study suggests that a stiffening process in the organic matrix component contributes to bone fragility independently of mineral content. Springer-Verlag 2009-09-12 2009-10 /pmc/articles/PMC2759010/ /pubmed/19756347 http://dx.doi.org/10.1007/s00223-009-9289-8 Text en © The Author(s) 2009
spellingShingle Article
Fratzl-Zelman, N.
Roschger, P.
Gourrier, A.
Weber, M.
Misof, B. M.
Loveridge, N.
Reeve, J.
Klaushofer, K.
Fratzl, P.
Combination of Nanoindentation and Quantitative Backscattered Electron Imaging Revealed Altered Bone Material Properties Associated with Femoral Neck Fragility
title Combination of Nanoindentation and Quantitative Backscattered Electron Imaging Revealed Altered Bone Material Properties Associated with Femoral Neck Fragility
title_full Combination of Nanoindentation and Quantitative Backscattered Electron Imaging Revealed Altered Bone Material Properties Associated with Femoral Neck Fragility
title_fullStr Combination of Nanoindentation and Quantitative Backscattered Electron Imaging Revealed Altered Bone Material Properties Associated with Femoral Neck Fragility
title_full_unstemmed Combination of Nanoindentation and Quantitative Backscattered Electron Imaging Revealed Altered Bone Material Properties Associated with Femoral Neck Fragility
title_short Combination of Nanoindentation and Quantitative Backscattered Electron Imaging Revealed Altered Bone Material Properties Associated with Femoral Neck Fragility
title_sort combination of nanoindentation and quantitative backscattered electron imaging revealed altered bone material properties associated with femoral neck fragility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2759010/
https://www.ncbi.nlm.nih.gov/pubmed/19756347
http://dx.doi.org/10.1007/s00223-009-9289-8
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